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G9136

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.75

product name

明胶 来源于猪皮肤, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

生物源

Porcine skin

品質等級

無菌

γ-irradiated

種類

Type A

產品線

BioXtra

形狀

lyophilized powder

包裝

pkg of 10 mg

技術

cell culture | mammalian: suitable

表面覆盖率

100‑200 μg/cm2

凝膠強度

~300 g Bloom

溶解度

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

運輸包裝

ambient

儲存溫度

room temp

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應用

建议将其按100-200 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用和研究目标。

明胶已用于多种应用。 它可用于对细胞培养器皿进行包被以改善细胞的附着,加入至PCR以稳定Taq DNA,作为Western blotting、ELISA和免疫化学中的封闭试剂,以及作为细菌学中用于物种分化培养基的组分。 作为一种生物相容性的聚合物,它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。在制药工业中,明胶可用作悬浮和包封剂,以及其他应用。

成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶,当在室温下储存于密闭容器中时,将可保持多年不变。 当在空气存在时在100℃下加热时,它会溶胀变软,并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂,如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数,可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。

本品为A型冻干粉,适用于细胞培养,凝胶强度为300。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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文章

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

癌症幹細胞培養基、球形板和癌症幹細胞標記,以培養和表徵 CSC 群。

條款

這份用於細胞培養的明膠塗佈方案提供了明膠類型、表面覆蓋濃度以及最佳化技巧等相關資訊。

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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