跳轉至內容
Merck
全部照片(2)

重要文件

G9284

Sigma-Aldrich

胍 盐酸盐 溶液

Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5

同義詞:

亚氨基脲 盐酸盐, 盐酸胍, 胍盐酸盐

登入查看組織和合約定價


About This Item

經驗公式(希爾表示法):
CH5N3 · HCl
CAS號碼:
分子量::
95.53
Beilstein:
3591990
MDL號碼:
分類程式碼代碼:
12352107
PubChem物質ID:
NACRES:
NA.55
暫時無法取得訂價和供貨情況

應用

盐酸胍溶液已用于:
  • 蛋白质变性
  • 对下丘脑、丘脑、前额叶皮层和脑干样本进行匀浆,提取总 β-淀粉样蛋白(Aβ)并进行 Aβ42 定量
  • 作为一种离液变性剂处理人类免疫缺陷病毒-1(HIV-1)样本,研究其对 HIV-1 敏感性的影响并测定蛋白质稳定性

生化/生理作用

盐酸胍(Gnd-HCl)是一种高效的离液剂(chaotropic agent),作为变性剂被广泛用于RNA分离、球蛋白变性和蛋白质复性研究。它是一种合适的样品缓冲剂,可用于亲和纯化的蛋白质复合物的快速有效的洗脱,以及之后的蛋白质消化步骤。[1]GnHCl可用于提取富含细胞包基质(ECM)‐的组织,包括骨骼、软骨、韧带和肌腱中的蛋白质。[2]Gnd-HCl不用于蛋白质组学实验,因为其在低摩尔浓度下会干扰胰蛋白酶活性并且在与十二烷基硫酸钠(SDS)结合时具有沉淀倾向。[1]它适用于进行快速和单步亲和纯化质谱(AP-MS)实验。

象形圖

Exclamation mark

訊號詞

Warning

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Jianwen Xu et al.
Journal of pharmaceutical sciences, 110(12), 3819-3828 (2021-09-11)
The purpose of this investigation was to highlight the utility of nuclear magnetic resonance (NMR) as a multi-attribute method for the characterization of therapeutic antibodies. In this case study, we compared results from isothermal chemical denaturation (ICD) and NMR with
Nitish Agrawal et al.
PloS one, 6(6), e21339-e21339 (2011-07-09)
The HIV-1 envelope glycoprotein (Env) spike is challenging to study at the molecular level, due in part to its genetic variability, structural heterogeneity and lability. However, the extent of lability in Env function, particularly for primary isolates across clades, has
Jessica Colby-Milley et al.
PloS one, 10(6), e0130177-e0130177 (2015-06-16)
In addition to cognitive decline, individuals affected by Alzheimer's disease (AD) can experience important neuropsychiatric symptoms including sleep disturbances. We characterized the sleep-wake cycle in the TgCRND8 mouse model of AD, which overexpresses a mutant human form of amyloid precursor
Yalda Ashraf Kharaz et al.
Proteomics, 17(13-14) (2017-05-27)
Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different
Jon W Poulsen et al.
Journal of proteome research, 12(2), 1020-1030 (2012-11-29)
Protein digestion is an integral part of the "shotgun" proteomics approach and commonly requires overnight incubation prior to mass spectrometry analysis. Quadruplicate "shotgun" proteomic analysis of whole yeast lysate demonstrated that Guanidine-Hydrochloride (Gnd-HCl) protein digestion can be optimally completed within

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務