推薦產品
形狀
lyophilized powder
比活性
25-75 units/mg protein
分子量
~128 kDa (by gel filtration)
成份
Protein, ≥60% biuret
儲存溫度
−20°C
一般說明
Research area: Cell Signaling
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
應用
Glycerokinase from Cellulomonas sp. has been used:
- for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
- to study the effect of sugar in fluorescence emission.
- in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.
生化／生理作用
甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸，并且是甘油利用中的限速酶。 它也受到果糖-1，6-二磷酸的反馈调节。
物理性質
等电点：4.2
米氏常数：4.4 x 10-5M（甘油），4.3 x 10-4M（ATP）
抑制剂：对对氯汞苯甲酸，重金属离子（Pb++，Fe++，Hg++，Ag+）
最佳pH：9.8（G-3-PDH系统），7.8（G-3-P氧化酶系统）最佳温度：500C
pH稳定性：pH 5.5 x 10.0 (25oC, 20hr)
热稳定性：低于40oC（pH 7.5，15min）
底物特异性：这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基，
形成sn-甘油-3-P。酶具有最高的
甘油特异性，也能磷酸化二羟基丙酮
和甘油醛（表1，2）。Mg++ 基本上是反应必需的。
.
米氏常数：4.4 x 10-5M（甘油），4.3 x 10-4M（ATP）
抑制剂：对对氯汞苯甲酸，重金属离子（Pb++，Fe++，Hg++，Ag+）
最佳pH：9.8（G-3-PDH系统），7.8（G-3-P氧化酶系统）最佳温度：500C
pH稳定性：pH 5.5 x 10.0 (25oC, 20hr)
热稳定性：低于40oC（pH 7.5，15min）
底物特异性：这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基，
形成sn-甘油-3-P。酶具有最高的
甘油特异性，也能磷酸化二羟基丙酮
和甘油醛（表1，2）。Mg++ 基本上是反应必需的。
.
單位定義
一个单位为在pH 9.8和25℃下，在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。
外觀
冻干粉含磷酸盐缓冲盐和葡萄糖酸钠
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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