The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.
推薦產品
生物源
bovine fetus
無菌
sterile-filtered
產品線
FBS Classic
成份
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
來源
USA origin
技術
cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable
雜質
≤10 EU/mL endotoxin
運輸包裝
dry ice
儲存溫度
−20°C
尋找類似的產品？ 前往 產品比較指南
一般說明
應用
準備報告
在56°C加热30分钟制备。
该产品是在56°C加热30分钟而制备的。将血清加热至56°C 30分钟可灭活补体系统以进行免疫测定。热激活还会使培养物中其他未确定的细胞生长抑制剂失活。
分析報告
- 内毒素和血红蛋白测试
- 检测了是否存在细菌、病毒和支原体
- 在无菌条件下用0.1微米膜过滤三次
也與該產品經常一起購買
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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文章
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
鈣是一種離子穩定的二價陽離子，在細胞培養中具有重要的有益和有毒特性。它是各種細胞培養基的成分之一。
回顾使用胎牛（或小牛）血清进行细胞培养的基础知识，以及采购和法规如何影响细胞测定所用血清的价格。
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
條款
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
使用儲存和解凍 FBS 及其他血清的最佳作法來保護您的投資。在您的細胞培養方案中使用 FBS 樣品進行驗證。
相關內容
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
探索我们广泛的胎牛血清(FBS)产品组合并根据您的实验室需求和预算选择合适的产品。了解全球事件如何影响FBS价格。
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
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Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?
1 answer-
Helpful?
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Recommended storage is listed as -20C; is it safe/ok to store at -80C?
1 answer-
The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
1 answer-
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
1 answer-
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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Does Sigma-Aldrich offer delipidated bovine serum?
1 answer-
At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
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