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F0392

Sigma-Aldrich

胎牛血清

USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

同義詞:

FBS, FCS, 血清

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
eCl@ss:
32160409
NACRES:
NA.71
暫時無法取得訂價和供貨情況

生物源

bovine fetus

無菌

sterile-filtered

產品線

FBS Select

形狀

liquid

成份

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

來源

USA origin

技術

cell culture | mammalian: suitable

雜質

≤10 EU/mL endotoxin

運輸包裝

dry ice

儲存溫度

−20°C

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應用

Fetal Bovine Serum has been used as a supplement for various cell culture techniques as it provides many non-defined growth promoting and survival enhancing factor to cells in culture.[1][2][3]

生化/生理作用

Fetal Bovine Serum (FBS) is a constituent of animal cell culture media. It contains hormones and growth factors.[4] FBS promotes in vitro cell culture, by providing binding proteins, lipids, minerals, attachment and spreading factors.[5]

準備報告

10,000 Molecular Weight Cut-off

分析報告

  • 内毒素和血红蛋白测试
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

The use of fetal bovine serum: ethical or scientific problem?
Jochems CEA, et al.
Alternatives to Laboratory Animals : ATLA, 30(2), 219-227 (2002)
Liver Tissue Engineering Based on Poly (ethylen Glycol) Hydrogels (2010)
Blocking anaplerotic entry of glutamine into the TCA cycle sensitizes K-Ras mutant cancer cells to cytotoxic drugs
Saqcena M, et al.
Oncogene, 34(20), 2672-2672 (2015)
Direct interactions with both p27 and Cdk2 regulate Spy1-mediated proliferation in vivo and in vitro
Al Sorkhy M, et al.
Cell Cycle, 15(1), 128-136 (2016)
Aisling M Redmond et al.
PloS one, 14(11), e0225180-e0225180 (2019-11-21)
HER2 is a transmembrane receptor tyrosine kinase, which plays a key role in breast cancer due to a common genomic amplification. It is used as a marker to stratify patients in the clinic and is targeted by a number of

文章

回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

查找有关FBS和其他血清产品的常见问题解答(FAQ)以及相关产品的链接。

條款

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

使用儲存和解凍 FBS 及其他血清的最佳作法來保護您的投資。在您的細胞培養方案中使用 FBS 樣品進行驗證。

相關內容

探索我们广泛的胎牛血清(FBS)产品组合并根据您的实验室需求和预算选择合适的产品。了解全球事件如何影响FBS价格。

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer
    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

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