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重要文件

E3763

Sigma-Aldrich

乙氧基试卤灵

≥98% (TLC), powder

同義詞:

7-乙氧基-3H-吩恶嗪-3-酮, O 7 -乙基试卤灵, 乙氧基试卤灵

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About This Item

經驗公式(希爾表示法):
C14H11NO3
CAS號碼:
分子量::
241.24
Beilstein:
225973
MDL號碼:
分類程式碼代碼:
12161501
PubChem物質ID:
NACRES:
NA.47
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品質等級

化驗

≥98% (TLC)

形狀

powder

儲存條件

(Tightly closed. Dry)

技術

activity assay: suitable

顏色

orange to red

mp

223-225 °C (lit.)

溶解度

chloroform: 9.80-10.20 mg/mL, clear, orange

適合性

suitable for fluorescence

儲存溫度

−20°C

SMILES 字串

CCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F

InChI

1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3

InChI 密鑰

ZOSYTBPPLWBBKM-UHFFFAOYSA-N

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一般說明

试卤灵被用作不同细胞色素 P (CYP) 亚型的标记物或底物。试卤灵乙醚是 3-甲基胆蒽处理动物的微粒体代谢的首选试卤灵乙醚。[1]
试卤灵(Resorufin)是刃天青的还原产物,也称为阿尔玛蓝(Alamar Blu)。[2]它在较长的波长下发射荧光,并且其羟基可进行灵活的修饰。[3]

适用研究领域:细胞信号传导[3]

應用

试卤灵乙醚适用于研究细胞色素 P450 同工酶 1A1 和 1A2 的脱烷基活性,其中芳基烷基和烷基异硫氰酸盐及其谷胱甘肽、半胱氨酸和 A′-乙酰半胱氨酸结合物用作抑制剂。[4]适合于研究细胞色素 P450 活性 (CYP1A1) 的乙氧基试卤灵-O-脱乙基酶 (EROD) 活性。[5]

基底

细胞色素 P450 连接酶 CXPIA1/2 的荧光底物

基底

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Reduction of resazurin to resorufin catalyzed by gold nanoparticles: dramatic reaction acceleration by laser or LED plasmon excitation
Alejo CJ, et al.
Catalysis Science & Technology, 1(8), 1506-1511 (2011)
C C Conaway et al.
Carcinogenesis, 17(11), 2423-2427 (1996-11-01)
A series of arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and N-acetylcysteine conjugates were used to study their inhibitory activity toward the dealkylation of ethoxyresorufin (EROD), pentoxyresorufin (PROD), and methoxyresorufin (MROD) in liver microsomes obtained from the 3-methylcholanthrene or
A resorufin derivative as a fluorogenic indicator for cell viability
Ishiyama M, et al.
Analytical Sciences, 15(10), 1025-1028 (1999)
T E M Parente et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 150(2), 252-260 (2009-05-23)
We investigated the presence and inducibility of CYP1A in suckermouth catfish (Hypostomus affinis and Hypostomus auroguttatus, Loricariidae), tilapia (Oreochromis niloticus, Cichlidae) and mice (Mus musculus, Muridae). Alkoxyresorufin-O-dealkylases (EROD, MROD, PROD and BROD) were detected and proved to be inducible (beta-naphthoflavone
Shotaro Uehara et al.
The Journal of pharmacology and experimental therapeutics, 339(2), 654-661 (2011-08-19)
The cynomolgus monkey is widely used as a primate model in preclinical studies because of its evolutionary closeness to humans. Despite their importance in drug metabolism, the content of each cytochrome P450 (P450) enzyme has not been systematically determined in

文章

Phase I biotransformation reactions increase drug compound polarity, mainly occurring in hepatic circulation.

第一階段生物轉化反應會增加藥物化合物的極性,主要發生在肝循環中。

Questions

  1. What is the solution stability of E3763?

    1 answer
    1. It is recommended to prepare solutions fresh.

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