E2520

Anti-Epidermal Growth Factor antibody, Mouse monoclonal

clone EGF-10, purified from hybridoma cell culture

• 同義詞: Anti-CEGF
• MDL號碼: MFCD00239669
• 分類程式碼代碼: 51111800
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: EGF-10, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 6 kDa
• 物種活性: human
• 技術: dot blot: 0.1 μg/mL using recombinant human EGF; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; radioimmunoassay: suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: P01133
• 應用: research pathology
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EGF(1950)

描述

一般說明

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

特異性

Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

免疫原

recombinant human EGF

應用

Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.

Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

生化／生理作用

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves