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Merck
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重要文件

D9154

Sigma-Aldrich

邻联茴香胺 二盐酸盐

tablet

同義詞:

3,3'-二甲氧基联苯胺 二盐酸盐, 固蓝 B

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About This Item

經驗公式(希爾表示法):
C14H16N2O2 · 2HCl
CAS號碼:
分子量::
317.21
Beilstein:
3917996
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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產品名稱

邻联茴香胺 二盐酸盐, tablet, 10 mg substrate per tablet

形狀

tablet

溶解度

water: 1 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI 密鑰

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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特異性

邻联茴香胺(3,3′-dimethoxybenzidine)是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物,颜色为黄橙色,可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。

警告

可能致癌。

重構

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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M Kaszuba et al.
Biochimica et biophysica acta, 1419(2), 221-228 (1999-07-17)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Cathrine Nordgaard et al.
International journal of molecular sciences, 22(17) (2021-09-11)
p38 and c-Jun N-terninal kinase (JNK) are activated in response to acute stress and inflammatory signals. Through modification of a plethora of substrates, these kinases profoundly re-shape cellular physiology for the optimal response to a harmful environment and/or an inflammatory
Rémy Ricoux et al.
Bioconjugate chemistry, 19(4), 899-910 (2008-03-08)
To develop artificial hemoproteins that could lead to new selective oxidation biocatalysts, a strategy based on the insertion of various iron-porphyrin cofactors into Xylanase A (Xln10A) was chosen. This protein has a globally positive charge and a wide enough active
Ozcan Erel
Clinical biochemistry, 38(12), 1103-1111 (2005-10-11)
To develop a new, colorimetric and automated method for measuring total oxidation status (TOS). The assay is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement

文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

條款

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

葡萄糖氧化酶活性透過 500 奈米的連續分光光度法測定,顯示葡萄糖氧化率。

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

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相關內容

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

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