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重要文件

D7876

Sigma-Aldrich

二胺氧化酶 来源于猪肾脏

≥0.05 unit/mg solid

同義詞:

胺:氧氧化还原酶(脱氨)(含吡哆醛)

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

Porcine kidney

形狀

solid

比活性

≥0.05 unit/mg solid

分子量

170 kDa

溶解度

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

異物活動

monoamine oxidase (benzylamine substrate) ≤1%

儲存溫度

−20°C

一般說明

猪肾来源的二胺氧化酶是由两个分子量均为87kDa的相同亚基组成的同二聚体,每个亚基含有一个吡哆醛磷酸分子和一个铜原子。[1]酶分子量为170kDa。[2]酶是一种糖蛋白,含有5%己糖、3.3%葡萄糖胺、2.6% N-乙酰葡萄糖胺和0.25% N-乙酰神经氨酸。酶对刀豆蛋白A具有高亲和力。[3]以尸胺和组胺作为底物时的最适pH为6.3-7.4。[1]

應用

来自猪肾的二胺氧化酶已被用于研究测定鸟氨酸脱羧酶活性的基于发光的测试。来自猪肾的二胺氧化酶也用于研究二胺氧化酶中 N-连接寡糖结构。
猪肾来源的二胺氧化酶已用于制备组胺生物传感器。[4]

生化/生理作用

二胺氧化酶催化单胺、二胺和组胺氧化成为醛、氨和过氧化氢。[5]该酶属于含铜的胺氧化酶,是氮代谢中的关键酶。[6]二胺氧化酶受到二乙基二硫代氨基甲酸、苯肼、氨基脲、氰化物、异烟酸肼抑制。[7]

單位定義

在 pH 7.2 和 37℃ 条件下,1 个单位每小时将氧化 1.0 μmol 腐胺。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Marta Moniente et al.
Food research international (Ottawa, Ont.), 160, 111735-111735 (2022-09-10)
Lentilactobacillus parabuchneri is the main bacteria responsible for the accumulation of histamine in cheese. The goal of this study was to assess the efficiency of potential histamine-degrading microbial strains or, alternatively, the action of the diamine oxidase (DAO) enzyme in
M A Shah et al.
The Biochemical journal, 253(1), 103-107 (1988-07-01)
Pig kidney diamine oxidase (DAO) was found to contain 5% (w/w) natural hexose, 3.25% glucosamine, 2.61% N-acetylglucosamine and 0.25% N-acetylneuraminic acid. The enzyme exhibited strong affinity towards concanavalin A (Con A) with a stoichiometry of 1:4.6. The kinetics of interaction
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of

條款

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

相關內容

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

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