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Merck
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重要文件

CWA-1069

Sigma-Aldrich

Anti-Human PD-L1 (29E.2A3) Colorwheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

同義詞:

Programmed cell death 1 ligand 1

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About This Item

分類程式碼代碼:
41116015
NACRES:
NA.41
暫時無法取得訂價和供貨情況

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

29E.2A3, monoclonal

產品線

ColorWheel®

形狀

lyophilized

分子量

calculated mol wt 33.28 kDa

純化經由

using Protein A

物種活性

human

物種活性(以同源性預測)

rat

包裝

antibody small pack of 25 μL

環保替代產品特色

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

flow cytometry: suitable

同型

IgG2bκ

表位序列

Extracellular domain

Protein ID登錄號

UniProt登錄號

相容性

for use with ColorWheel® Dyes (Required, sold separately)

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

基因資訊

mouse ... CD274(29126)

一般說明

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

特異性

Clone 29E.2A3 is a mouse monoclonal antibody that detects human PD-L1 (CD274). It targets an epitope within the extracellular domain.

免疫原

Full-length recombinant human PD-L1.

應用

Quality Control Testing

Evaluated by Flow cytometry in PHA-stimulated human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million PHA-stimulated Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1069, Anti-Human PD-L1 (29E.2A3) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel® Dye (Sold Separately)

標靶描述

Programmed cell death 1 ligand 1 (UniProt: Q9NZQ7; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, CD274) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID: 29126) in human. PD-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form that contains a large extracellular (aa 19-238) with an Ig-like V-type domain (aa 19-127) and an Ig-like C2-type domain (aa 133-225), followed by a transmembrane domain (aa 239-259) and a cytoplasmic tail (aa 260-290). PD-L1 is expressed on activated T- and B-cells, dendritic cells, keratinocytes, and monocytes. Higher expression has also been reported in heart, skeletal muscle, placenta, and lung. It plays a critical role in induction and maintenance of immune tolerance to self. When engaged together with the TCR, the interaction of PD-1 with its ligands, PD-L1 and PD-L2, deliver an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions.

外觀

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重構

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

法律資訊

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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利用ColorWheel®流式细胞术抗体及染料的灵活性,自如设计多重或多色流式细胞分析方案,简化多重流式细胞分析流程。

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ColorWheel®流式试剂3步法制备方案,让您在流式细胞术中体验到超强简便性。采用ColorWheel®流式抗体和染料,可轻松配制理想的流式细胞分析试剂,全程只需不到5分钟的实操时间。

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采用ColorWheel®流式抗体和染料,解锁流式细胞术检测的设计自由。ColorWheel®流式家族简单灵活,在保证实验成功率的同时,可帮助简化实验室流式细胞分析流程。

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