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Merck
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重要文件

CWDS-MF375

Sigma-Aldrich

ColorWheel® Antibody-Ready mFluor UV375 Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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About This Item

分類程式碼代碼:
41116015
NACRES:
NA.32
暫時無法取得訂價和供貨情況

產品線

ColorWheel®

品質等級

形狀

lyophilized

環保替代產品特色

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

flow cytometry: suitable

螢光

λex 351 nm; λem 387 nm

相容性

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

mFluor UV375 succinimidyl esters dye is well suited for multicolor flow cytometry applications. It exhibits a relatively larger Stokes shift and exhibit an excitation max at 351 nm and emission max at 387 nm. These spectral properties make it an ideal alternate to BUV395. However, in contrast to BUV395, which is a polymer-based dye, mFlour UV375 is a small organic molecule that case be conjugated to antibodies with ease. The NHS esters can react readily with the primary amines in proteins, amine-modified oligonucleotides, and other amine-containing molecules and the resulting dye conjugates exhibit good stability.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

應用

Quality Control Testing:

Flow Cytometry: Excitation: 351 nm, Emission: 387 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

外觀

CWDS-MF375-25UL Set Components

CWD-MF375-25UL - ColorWheel® Antibody-Ready mFluor UV375 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

重構

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律資訊

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

11 - Combustible Solids


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查阅专家针对ColorWheel®流式抗体和染料常见问题(FAQ)所作的解答。

利用ColorWheel®流式细胞术抗体及染料的灵活性,自如设计多重或多色流式细胞分析方案,简化多重流式细胞分析流程。

條款

ColorWheel®流式试剂3步法制备方案,让您在流式细胞术中体验到超强简便性。采用ColorWheel®流式抗体和染料,可轻松配制理想的流式细胞分析试剂,全程只需不到5分钟的实操时间。

相關內容

采用ColorWheel®流式抗体和染料,解锁流式细胞术检测的设计自由。ColorWheel®流式家族简单灵活,在保证实验成功率的同时,可帮助简化实验室流式细胞分析流程。

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