tsa201 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96121229, human kidney, Epithelial-like

• 同義詞: tsA-201 Cells
• 分類程式碼代碼: 41106514

屬性

• 生物源: human kidney
• 包裝: tube of 5 μg 96121229-DNA-5UG; pkg of vial of cells 96121229-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human embryonal kidney, SV40 transformed

細胞系描述

tsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.

應用

tsa201 human cell line has been used for protein-binding studies,[1] whole-cell patch clamp recordings[2] and transfection studies.[3]

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO₂; 37°C. Do not let cells become over confluent as this can affect morphology.

其他說明

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