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重要文件

TM3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

分類程式碼代碼:
41106514
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生物源

mouse testis

包裝

tube of 5 μg 91060526-DNA-5UG
pkg of vial of cells 91060526-1VL

增長模式

Adherent

染色體組型

Not specified

形態學

Not specified

產品

Prostaglandin F2a

受體

Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse BALB/c testis, Leydig cell

細胞系描述

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

應用

Hormone studies

培養基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37²C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Lot/Batch Number

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