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重要文件

CA200

Sigma-Aldrich

cAMP酶免疫检测试剂盒, 直接法

同義詞:

cAMP detection kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

技術

ELISA: suitable

品質等級

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

腺苷 3′,5′-环单磷酸(cAMP)是许多生理过程的第二信使。在腺苷酸环化酶存在的条件下,由三磷酸腺苷(ATP)合成。[1]
非放射性、竞争性酶免疫测定,用于定量1M HCL处理组织和细胞培养液中的总cAMP含量。cAMP试剂盒采用cAMP多克隆抗体竞争性结合样品内的cAMP或共价连接cAMP的碱性磷酸酶分子。

應用

cAMP 酶免疫测定试剂盒(直接法)可用于测量细胞培养基[2] 中的cAMP水平,适用毒性和减毒巨噬细胞。 [3] 也用于定量嗅觉上皮细胞中的cAMP合成。[4]
有关详情,请参阅随附 方案

生化/生理作用

研究显示,腺苷3′,5′-环单磷酸(cAMP)参与心血管和神经系统、免疫机制、细胞生长分化以及一般代谢等多种生理过程。 破解调节途径中的cAMP调控机制可能有助于理解许多疾病状态的生理学和病理学。[1]

其他說明

试剂开口前须在室温下预热30分钟。

僅套裝組件

產品號碼
描述

  • 0.1 M Hydrochloric Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Direct cAMP Assay Layout Sheet 1 x 1

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

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訊號詞

Danger

危險分類

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

標靶器官

Respiratory system

安全危害

儲存類別代碼

3 - Flammable liquids

閃點(°F)

120.2 °F

閃點(°C)

49 °C


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