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Merck
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C9658

Sigma-Aldrich

羧肽酶  G 来源于假单胞菌

lyophilized powder, ≥3 units/mg protein

同義詞:

γ-谷氨酰水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

≥3 units/mg protein

分子量

homodimer ~90 kDa

成份

Protein, ~70% biuret

溶解度

H2O: soluble 0.5 mg/mL

儲存溫度

−20°C

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相關類別

應用

来自假单胞菌属或 γ-谷氨酰基水解酶的羧肽酶 G 已用于研究中评估假单孢氧还蛋白 COOH-末端在 P-450cam(细胞色素 m)羟基化中的作用。来源于假单胞菌属的羧肽酶 G 也被用于研究肺腺癌细胞中一氧化氮通过 NO‑cGMP 信号通路对培美曲塞细胞毒性的产生影响。

生化/生理作用

羧肽酶 G 是溶酶体,巯基依赖性蛋白酶,其逐渐切割 γ-谷氨酰蝶酰多聚-γ-谷氨酸,产生蝶酰-α-谷氨酸(叶酸)和游离谷氨酸。它被认为对 γ-谷氨酰基键具有高度特异性,但对于离去基团的 C-末端氨基酸则不具有高度特异性。该同型二聚体的分子量约为 90kDa。酶被 Zn2+ 离子激活。

單位定義

在 pH7.3,30℃ 下,一个单位的酶每分钟将(+)氨甲蝶呤水解产生 1.0 μmole L-谷氨酸。

外觀

含有醋酸钠盐。

準備報告

溶液应现配现用。
色谱纯化产品

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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