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重組細胞
expressed in E. coli
形狀
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
濃度
14 mg/mL
UniProt登錄號
儲存溫度
−20°C
基因資訊
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
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應用
来自铜绿假单胞菌的酰胺酶已被用于测试其水解曲毒素A的能力。[1]
这些水解酶对于生物技术的重要性正在迅速增加,因为它们的潜在应用遍及化学和制药行业以及生物修复。 固定化酰胺酶可被有效地用于从丙烯酰胺产生丙烯酸,从而将有毒的环境污染物转化为广泛使用的工业原材料。酰胺酶是治疗人类免疫缺陷病毒和疟疾的一种潜在方法。 它们可用于消除废水中的金属离子[2]。
生化/生理作用
来自铜绿假单胞菌的酰胺酶是一种6 × 38-kDa的酶,可催化小范围短脂族酰胺的水解。 每种酰胺酶单体都是由一种带有额外81个残基长C末端区域的球形四层αββα三明治结构域所组成的[2]。
單位定義
在pH 7.2、37 °C下,一单位可在每分钟内将1.0 μmol的乙酰胺和羟胺转化为乙酰氧肟酸酯和氨。
外觀
含有7 mM 2-巯基乙醇和磷酸盐缓冲液的50%甘油溶液
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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