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重要文件

C7527

Sigma-Aldrich

氯化胆碱

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

同義詞:

(2-羟乙基)三甲基氯化铵

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About This Item

線性公式:
(CH3)3N(Cl)CH2CH2OH
CAS號碼:
分子量::
139.62
Beilstein:
3563126
EC號碼:
MDL號碼:
分類程式碼代碼:
12352205
PubChem物質ID:
NACRES:
NA.75
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生物源

synthetic (organic)

產品線

BioReagent

化驗

≥98%

形狀

crystals

分子量

Mw 139.62 g/mol

技術

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

顏色

white

pH值

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

溶解度

H2O: 50 mg/mL

SMILES 字串

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI 密鑰

SGMZJAMFUVOLNK-UHFFFAOYSA-M

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一般說明

氯化胆碱是一种季铵盐,与氢键供体(HBD)缔合。[1]它是许多重要生理功能必不可少的成分,用作营养添加剂。胆碱是甲基转移反应的甲基来源。[2]

應用

氯化胆碱已用于:
  • 用作胆碱乙酰基转移酶 (ChAT)缓冲液组分,用于培养人成神经细胞瘤(SK-N-SH)细胞中的胆碱吸收[3]
  • 生成胆碱标准曲线,以定量磷脂酶D (PLD)活性[4]
  • 用作解剖内海马脑切片的切片溶液组分[5]

基底

胆碱乙酰基转移酶底物

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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存取文件庫

Synthesis and characterization of choline chloride based binary mixtures
Abbas Q and Binder L
ECS Transactions, 33(7), 49-59 (2010)
Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation
Carou MC, et al.
Acta Histochemica, 119(5), 462-470 (2017)
Choline: an essential nutrient for public health
Zeisel SH and Da Costa KA
Nutr. Rev, 67(11), 615-623 (2009)
Harvie P Portugaliza et al.
Scientific reports, 9(1), 14595-14595 (2019-10-12)
Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of
Determination of high-affinity choline uptake (HACU) and choline acetyltransferase (ChAT) activity in the same population of cultured cells
Ray B, et al.
Brain Research, 1297(11), 160-168 (2009)

Questions

1–3 of 3 Questions  
  1. Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

    1 answer
    1. This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.

      Helpful?

  2. Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

    1 answer
    1. This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w

      Helpful?

  3. Hi, could you please provide the product information sheet for Choline chloride.

    1 answer
    1. There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:

      Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf

      Certificate of Origin
      https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V

      Helpful?

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