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重要文件

C4018

Sigma-Aldrich

胆酰甘氨酸水解酶 来源于产气荚膜梭菌(韦氏梭菌)

lyophilized powder, ≥100 units/mg protein

同義詞:

3α,7α,12α-三羟基-5β-氯烷-24-甲酰甘氨酸酰胺水解酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
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形狀

lyophilized powder

比活性

≥100 units/mg protein

成份

Protein, ≥50% biuret

運輸包裝

wet ice

儲存溫度

−20°C

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應用

来自 Sigma 的酶已在各种研究中用于胆汁样品的分析。[1][2][3]

生化/生理作用

该酶是一种 N-末端亲核 (Ntn) 水解酶,催化酰胺键水解,从类固醇核心释放甘氨酸/牛磺酸部分,最终生成非结合胆汁酸。已证明可氧化巯基的药物(例如,对汞苯甲酸盐、碘乙酰胺、Hg 2 + 、Cu 2 + 和 Cd 2 + )可强烈抑制 产气荚膜梭菌的胆盐水解酶 (BSH) 活性 [4]

單位定義

一个单元将在37°C、pH 5.6 条件下5 min 内将 1.0μmol 甘胆酸水解为甘氨酸和胆酸。

外觀

部分纯化的冻干粉末,含有缓冲盐和稳定剂

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Akira Honda et al.
Journal of lipid research, 46(2), 287-296 (2004-12-04)
Cerebrotendinous xanthomatosis (CTX), sterol 27-hydroxylase (CYP27A1) deficiency, is associated with markedly reduced chenodeoxycholic acid (CDCA), the most powerful activating ligand for farnesoid X receptor (FXR). We investigated the effects of reduced CDCA on FXR target genes in humans. Liver specimens
Younghoon Kim et al.
Bioscience, biotechnology, and biochemistry, 72(6), 1483-1490 (2008-06-10)
This study characterizes the factors responsible for the cholesterol reduction by Lactobacillus acidophilus ATCC 43121. In addition, two-dimensional gel electrophoresis (2-DE) and protein profiling was also used to study the response of ATCC 43121 at the proteome level in the
Anil K Patel et al.
Applied biochemistry and biotechnology, 162(1), 166-180 (2009-08-12)
Probiotic has modernized the current dietetic sense with novel therapeutic and nutritional benefits to the consumers. The presence of bile salt hydrolase (BSH) in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol
Bile salt hydrolase activity in probiotics.
Máire Begley et al.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)

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