B8291

BSK-H Medium, Complete

With 6% rabbit serum, liquid, sterile-filtered, suitable for cell culture

• 同義詞: Barbour-Stoenner-Kelly medium, Borrelia burgdorferi medium
• 分類程式碼代碼: 12352207
• NACRES: NA.75

屬性

• 產品名稱: BSK-H Medium, Complete, sterile-filtered, with 6% rabbit serum, suitable for Borrelia burgdorferi
• 無菌: sterile-filtered
• 形狀: liquid
• 適合性: suitable for Borrelia burgdorferi
• 應用: food and beverages
• 成分: NaHCO₃: 2.068 g/L; phenol red: 0.01997 g/L; glucose: 5.64 g/L (Dextro); HEPES: 5.64 g/L
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

應用

Complete BSK-H Medium was used to culture B. burgdorferi to study the pathology of lyme borreliosis.[1][2] It may be used in serum supplemented formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi.

生化／生理作用

BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.[3]

準備報告

BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable