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B4500

Sigma-Aldrich

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐

trypsin substrate, chromogenic, ≥97% (HPLC), powder

同義詞:

BAEE

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About This Item

經驗公式（希爾表示法）:
C15H22N4O3 · HCl
CAS號碼:
2645-08-1
分子量：:
342.82
Beilstein:
3781694
EC號碼:
220-157-0
MDL號碼:
MFCD00012579
分類程式碼代碼:
12352204
PubChem物質ID:
24891777
NACRES:
NA.32

product name

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐, trypsin substrate

化驗

≥97% (HPLC)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI 密鑰

HIXDELXKSSLIKB-YDALLXLXSA-N

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一般說明

Nα-苯甲酰基-L-精氨酸乙酯盐酸盐是胰蛋白酶底物。是用于光谱测定的非蛋白质底物。

應用

  • N

  • α-苯甲酰基-L-精氨酸乙酯盐酸盐作为底物，用于：
  • 在牙龈卟啉单胞菌（ P. gingivalis ）囊泡中测定肽基精氨酸脱亚氨酶（PPAD）
  • 测定番木瓜植物部位酶提取物的蛋白水解活性
  • 测定大鼠尾肌腱束的胰蛋白酶活性

基底

胰蛋白酶的原型底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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存取文件庫

Chensong Pan et al.
Analytical and bioanalytical chemistry, 387(1), 193-204 (2006-11-07)
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is widely used in a variety of fields because it has the characteristics of speed, ease of use, high sensitivity, and wide detectable mass range for obtaining molecular weights and for structural characterization of
Ying-Qing Yu et al.
Analytical chemistry, 75(21), 6023-6028 (2003-11-01)
Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS solubilizes proteins without inhibiting trypsin or other common endopeptidases activity. Trypsin activity
Kinetic and Structural Differentiation of Trypsin from Different Origins
Aithal M, et al.
BioPharm International, 30(1), 38-+-38-+ (2017)
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
查看所有相關論文

文章

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

木瓜蛋白酶、半胱氨酸蛋白酶、特性與產品

發現半胱氨酸蛋白酶木瓜蛋白酶的特性和應用，並找到抑制劑、底物和其他木瓜蛋白酶產品。

條款

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

胰蛋白酶測試程序 (EC 3.4.21.4)

使用 BAEE 底物的連續分光光度速率測定方法可測量胰蛋白酶的活性，對於酵素的特性分析非常重要。

相關內容

Enzymatic Assay of Trypsinogen

Protocol for the enzymatic Trypsinogen Assay

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

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