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A9528

Sigma-Aldrich

两性霉素B，可溶

powder, suitable for cell culture, BioXtra

同義詞:

两性霉素B 来源于链霉菌 属, Fungizone

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About This Item

經驗公式（希爾表示法）:
C47H73NO17
CAS號碼:
1397-89-3
分子量：:
924.08
Beilstein:
78342
MDL號碼:
MFCD00877763
分類程式碼代碼:
12352207
eCl@ss:
34010400
PubChem物質ID:
24891468
NACRES:
NA.76
暫時無法取得訂價和供貨情況

產品名稱

两性霉素B，可溶, powder, γ-irradiated, BioXtra, suitable for cell culture

品質等級

300

無菌

γ-irradiated

產品線

BioXtra

形狀

powder

技術

cell culture | mammalian: suitable

抗生素活性譜

fungi
yeast

作用方式

cell membrane | interferes

儲存溫度

2-8°C

SMILES 字串

C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O

InChI

1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1

InChI 密鑰

APKFDSVGJQXUKY-INPOYWNPSA-N

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相關類別

一般說明

化学结构：多烯

應用

两性霉素B是来自链霉菌属的一种多烯抗真菌抗生素。它对真菌和细菌细胞膜的固醇，尤其是麦角固醇具有很高的亲和力。 维持抗真菌细胞培养的正常用量为2.5 mg/L，同时在培养基中使用青霉素和链霉素。

生化／生理作用

两性霉素B可与甾醇结合，在膜中形成孔，并导致小分子的泄漏。该产品对真菌和酵母最有效。

包裝

可溶性两性霉素B包装在琥珀色玻璃瓶中，内容物置于插入的熔锥内。

成分

本品为两性霉素 B 的胶体混悬液，以去氧胆酸盐为增溶剂。 处方为约 45% 两性霉素 B 和 35% 脱氧胆酸钠的磷酸钠和氯化钠溶液。

注意

两性霉素 B 在 37°C 培养 3 天仍保持活性。在-20°C 条件下，通过防风避光，本品可保持活性 5 年。

準備報告

两性霉素 B 可溶于水 (19.6-20.4 mg/mL)，在自然 pH 下，无需加热，即可得到淡黄色澄清至轻微浑浊的溶液。
水溶液由于溶解性差，不能过滤除菌。 如果在无菌水中复溶，则无需过滤除菌。

也可溶于 DMSO (30-40 mg/mL) 和二甲基甲酰胺 (2-4 mg/mL)。

其他說明

保存于密闭容器内，置于干燥通风处。本品对光和湿度敏感。置于干燥处保存。
根据 UV 纯度计算包装产品的实际固体重量。

僅套裝組件

產品號碼
描述
  • Amphotericin B ~ 45 %
  • Sodium deoxycholate ~ 35 %
  • Sodium phosphate balance

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H372

危險分類

STOT RE 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000102681
0000102681
0000416976
0000416975
0000416976

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存取文件庫

Derayvia Grimes et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24403-24414 (2020-09-16)
Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma-membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2, and ORAI3
Devin Tran et al.
Cornea, 38(7), 901-904 (2019-04-19)
Fungal infections in lamellar keratoplasty are a growing concern. Optisol-GS does not contain an antifungal agent and supplementation with 0.255 μg/mL Amphotericin B (AmpB) has been considered. This study tested the ability of 0.255 μg/mL AmpB in Optisol-GS to eliminate
Etienne Loire et al.
Heliyon, 6(10), e05146-e05146 (2020-10-22)
Contagious caprine pleuropneumonia is an infectious and contagious disease affecting goats and wildlife ruminants, mostly in Africa and Asia. It is caused by a mycoplasma, Mycoplasma capricolum susbp. capripneumoniae, which is very fastidious. This may be the reason why there
Ziying Yan et al.
Current protocols in microbiology, 58(1), e107-e107 (2020-07-09)
Human bocavirus 1 (HBoV1) is a small DNA virus that belongs to the Bocaparvovirus genus of the Parvoviridae family. HBoV1 is a common respiratory pathogen that causes mild to life-threatening acute respiratory tract infections in children and immunocompromised individuals, infecting
Malav S Trivedi et al.
The Journal of nutritional biochemistry, 25(10), 1011-1018 (2014-07-16)
Dietary interventions like gluten-free and casein-free diets have been reported to improve intestinal, autoimmune and neurological symptoms in patients with a variety of conditions; however, the underlying mechanism of benefit for such diets remains unclear. Epigenetic programming, including CpG methylation
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文章

Antifungals Reagents in Cell Culture

Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.

細胞培養中的抗真菌試劑

防止細胞培養物中的真菌、酵母菌和霉菌污染。透過廣泛的 Sigma® 抗真菌劑系列，找出最適合您的培養物的抗真菌劑。

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

抗生素殺菌曲線

抗生素致死曲線是一種劑量反應實驗，哺乳類動物細胞在選擇抗生素時，其劑量會不斷增加。

Questions

1–6 of 6 Questions  

  1. what is the potency of Amphotericin B solubilized solution?

    1 answer

    1. This is a powdered product. The potency in solution is dependent upon the concentration at which it is prepared. The minimum potency specification for this product is 240 µg/mg. The lot specific value will be reported on the Certificate of Analysis. Please see the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9528-5G0000247193.pdf

      Helpful?

  2. How much DSMO do I dissolve the 100mg powder in to achieve a stock concentration of 2.5mg/mL? 

    1 answer

    1. Amphotericin B is soluble in DMSO at 30-40 mg/mL. Add 100 mg powder to 40 mL DMSO in order to achieve a 2.5 mg/mL concentration.

      Helpful?

  3. What is the shelf life of Product A9528, Amphotericin B solubilized?

    1 answer

    1. Product No. A9528 has a recommended retest date of 5 years from the initial QC release date.  This date can be found on the lot-specific Certificate of Analysis.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the amount of Amphotericin B in Product No. A9528?

    1 answer

    1. The package size is the amount of Amphotericin B present in the vial.  If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.

      Helpful?

  6. What concentration should Product A9528, Amphotericin B solubilized, be used at?

    1 answer

    1. We recommend to use Product No. A9528 at 5.6 mg solid per Liter.  Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC.  The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.

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