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重要文件

A7512

Sigma-Aldrich

N-乙酰基-DL-苯丙氨酸 β-萘酯

≥98% (TLC), suitable for ligand binding assays

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About This Item

經驗公式(希爾表示法):
C21H19NO3
CAS號碼:
分子量::
333.38
MDL號碼:
分類程式碼代碼:
12352209
PubChem物質ID:
NACRES:
NA.26
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產品名稱

N-乙酰基-DL-苯丙氨酸 β-萘酯,

化驗

≥98% (TLC)

形狀

powder

技術

ligand binding assay: suitable

顏色

white

儲存溫度

2-8°C

SMILES 字串

CC(=O)NC(Cc1ccccc1)C(=O)Oc2ccc3ccccc3c2

InChI

1S/C21H19NO3/c1-15(23)22-20(13-16-7-3-2-4-8-16)21(24)25-19-12-11-17-9-5-6-10-18(17)14-19/h2-12,14,20H,13H2,1H3,(H,22,23)

InChI 密鑰

BBXRRTJNJCPGBU-UHFFFAOYSA-N

生化/生理作用

N-乙酰基-DL-苯丙氨酸β-萘酯(NAPBNE)是一种发色底物,用于鉴别、区分和表征丝氨酸蛋白酶和肽酶。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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M A Jongsma et al.
Analytical biochemistry, 212(1), 79-84 (1993-07-01)
An improved, time efficient, visual assay for quantitative determination of proteinase inhibitor activity in protein extracts is reported. Proteinase inhibitor activity of mammalian, bacterial, and fungal serine proteinases can be quantified. The method relies on radial diffusion of proteinase inhibitor
P Collin-Osdoby et al.
Molecular & general genetics : MGG, 243(6), 674-680 (1994-06-15)
Mutations at the apeA locus in Salmonella typhimurium lead to loss of a soluble enzyme ("protease I") that hydrolyzes the chromogenic endoprotease substrate N-acetyl phenylalanine beta-naphthyl ester. We have isolated pseudorevertants of S. typhimurium apeA mutations that have regained the
H Y Darani et al.
Parasitology, 115 ( Pt 3), 237-247 (1997-09-23)
A cationic Schistosoma mansoni cercarial antigen was shown to be a serine protease as it was capable of hydrolysing N-acetyl-DL-phenylalanine beta-naphthyl ester (NAPBNE) after precipitation by immunoelectrophoresis, and this reaction was modulated by the serine protease inhibitors phenylmethanesulfonyl fluoride (PMSF)
Miri Aharon et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 22(8), 847-852 (2002-10-25)
This study investigates the possible involvement of serine proteases in interferon-gamma (IFN-gamma) activity on WISH cells. It was observed that inhibition of (3)H-thymidine incorporation induced by IFN-gamma was abrogated by the serine protease inhibitors Nalpha-tosyl-L-lysyl-chloromethane and soybean trypsin inhibitor, both
H Y Darani et al.
Parasitology, 135(4), 467-472 (2008-01-23)
An enzyme found previously in extracts of adult Schistosoma mansoni worms, that hydrolysed the chromogenic substrate N-acetyl-DL-phenylalanine beta-naphthyl-ester, has here been further investigated and characterized. Evidence that the molecule found in the parasite was antigenically and enzymatically homologous with a

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