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重要文件

A6306

Sigma-Aldrich

琼脂水解酶 来源于大西洋假单胞菌

lyophilized powder, ≥5,000 units/mg protein (Lowry)

同義詞:

β-琼脂水解酶, 琼脂糖 4-溶菌酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42040102
NACRES:
NA.26
暫時無法取得訂價和供貨情況

生物源

bacterial (Pseudomonas atlantica)

品質等級

形狀

lyophilized powder

比活性

≥5,000 units/mg protein (Lowry)

儲存溫度

2-8°C

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相關類別

單位定義

一个单位将在40°C 、 pH 6.0 下每分钟从琼脂产生 1.0μg 还原糖(以 D -半乳糖测量)。

外觀

产品由磷酸盐缓冲盐、牛血清白蛋白和琼胶酶配制而成。 总蛋白质含量范围在10 – 30% w/w之间。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Ji-Hwan Seok et al.
Biotechnology letters, 34(6), 1041-1049 (2012-02-09)
α-Agarase hydrolyzes the α-1,3 linkage of agarose yielding agaro-oligosaccharides. It is less well characterized than β-agarase. AgaA gene (2.3 kb ORF), encoding the α-agarase from Thalassomonas JAMB A33, was subcloned into both a constitutive and an inducible expression vector. Both
Hyeok-Jin Ko et al.
Applied and environmental microbiology, 78(9), 3051-3058 (2012-02-22)
Autotransporters have been employed as the anchoring scaffold for cell surface display by replacing their passenger domains with heterologous proteins to be displayed. We adopted an autotransporter (YfaL) of Escherichia coli for the cell surface display system. The critical regions
Michael-Paul Vockenhuber et al.
Microbiology (Reading, England), 158(Pt 2), 424-435 (2011-11-15)
Transcriptional regulation of primary and secondary metabolism is well-studied in Streptomyces coelicolor, a model organism for antibiotic production and cell differentiation. In contrast, little is known about post-transcriptional regulation and the potential functions of small non-coding RNAs (sRNAs) in this
Young Hoon Oh et al.
Journal of microbiology and biotechnology, 21(8), 818-821 (2011-08-31)
An agar-degrading bacterium was isolated from the guts of spiny turban shells. It was identified as a Pseudoalteromonas species and named Pseudoalteromonas sp. JYBCL 1. The viscosity of the inoculated agar medium decreased by more than 60% after 20 h
Yuji Hatada et al.
Marine biotechnology (New York, N.Y.), 13(3), 411-422 (2010-08-06)
A gene of unknown function from the genome of the agar-degrading deep-sea bacterium Microbulbifer thermotolerans JAMB-A94(T) was functionally identified as a ι-carrageenase gene. This gene, designated as cgiA, is located together with two β-agarase genes, agaA and agaO in a

Questions

  1. Will this enzyme work in water or PBS, or must it be a potassium buffer (for which not further details are provided in the spec sheet)?

    1 answer
    1. Potassium buffer is not required. For assay purposes, this product is reconstituted in potassium phosphate buffer, pH 6.0, at 0.2 mg/ml, Solutions may also be prepared 40 mM Tris HCL, pH 7.5, containing 50 mM NaCl and 50% glycerol. This stock may be stored for extended periods at –20 °C. Other sources indicate that this enzyme may also be prepared in water containing 0.01% BSA and TBE buffer. These options have not been validated. Please see the link below to review a publication that may be of interest:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817930/

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