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重要文件

A3157

Sigma-Aldrich

阿尔新蓝 8GX

Dye content, ≥50%, certified by the Biological Stain Commission, powder

同義詞:

国固蓝 1, 阿尔新蓝

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About This Item

經驗公式(希爾表示法):
C56H68Cl4CuN16S4
CAS號碼:
分子量::
1298.86
顏色索引編號:
74240
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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產品名稱

阿尔新蓝 8GX, certified by the Biological Stain Commission

等級

certified by the Biological Stain Commission

品質等級

形狀

powder

成份

Dye content, ≥50%

技術

microbe id | staining: suitable

顏色

dark blue

溶解度

water: 1 mg/mL, blue

εmax

195-400 at 617-630 nm

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI 密鑰

CKLBXIYTBHXJEH-UHFFFAOYSA-J

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應用

阿尔新蓝8GX(Alcian Blue 8GX )已可用于小鼠MPC(间充质祖细胞)结节中硫酸化蛋白多糖的染色。[1]它已可用于牛关节软骨细胞中硫酸化蛋白多糖的染色。[2]

生化/生理作用

阿尔新蓝8GX是一种阳离子染料。它与酸性糖胺聚糖形成不溶性复合物,从而有助于糖胺聚糖的定量测定。[3]它还被用于骨和软骨的染色。[4]

適合性

经认证可用于Mowry′s pH 2.5方法、Scott′s pH 5.7方法以及Kreyberg′s方法中的角蛋白和粘蛋白染色。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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文章

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sigma 提供 EZBlue™ 和 ProteoSilver™ 用於蛋白質可視化的試劑,適用於蛋白質組學和傳統 PAGE 格式。

Questions

  1. Can the A9186 Alcian blue 8GX be used for the same assay as the A3157 Alcian blue 8GX previously used for mice spinal cord histology (frozen sections)?

    1 answer
    1. A9186 is intended to demonstrate glycoproteins when performing electrophoresis, while A3157 is intended for use with tissue sections. It is then tested on paraffin sections of small intestine, trachea, and larynx using different methods. Since A9186 was not tested for use with tissue sections, it is possible that it might not stain as well as product A3157, which was performance tested on paraffin sections. The same principle applies to other Alcian Blue 8GX products that are not tested for specific applications. It is advised to try using A9186, as the staining may be acceptable. If the staining is not acceptable, it is recommended to consider ordering A3157.

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