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重要文件

A2237

Sigma-Aldrich

磷酸酶,碱性虾

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

同義詞:

磷酸酶(碱性) 来源于虾, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
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重組細胞

expressed in proprietary host

品質等級

形狀

buffered aqueous glycerol solution

濃度

≥900 DEA units/mL

異物活動

RNAse, DNAse, none detected

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

應用

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.[1] It also may be used in pasteurization process for milks used in dairy products.[2]

生化/生理作用

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

單位定義

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

外觀

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

分析報告

Protein determined by biuret

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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