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重要文件

A1227

Sigma-Aldrich

ABTS 增强子

liquid, for peroxidase detection

同義詞:

2,2′-双(3-乙基苯并噻唑啉-6-磺酸)增强剂

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83
暫時無法取得訂價和供貨情況

產品名稱

ABTS 增强子, for peroxidase detection

品質等級

形狀

liquid

儲存溫度

2-8°C

應用

2,2′-次偶氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)已被用于测定杂色栓菌(Trametes versicolor)的漆酶活性。[1]它适用于酶联免疫吸附测定(ELISA),用于检测细胞发出的信号强度。[2]

生化/生理作用

2,2′-次偶氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)是一种非酚类底物。[3]它可能具有抗氧化性能。[4]添加化学增强剂可改善辣根过氧化物酶底物ABTS的检测。ABTS增强剂可将氧化产物的吸光度提高50-150%。这种增加作用可用于提高测定的灵敏度或节省测定成分。

外觀

以 11X 浓缩液形式提供

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Carc. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Mark E Levenstein et al.
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Journal of Plant Biochemistry & Physiology (2018)
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Molecular vision, 17, 2769-2775 (2011-11-09)
To evaluate total antioxidant status (TAS) in the plasma of pseudoexfoliation glaucoma (PEG) patients and to compare this level with a matching control group. Additionally, we aim to investigate the effect of the combined action of the lysyl oxidase-like 1
Manickam Naresh Kumar et al.
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Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification
Meng-Hsuan Wu et al.
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Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show

文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

1–3 of 3 Questions  
  1. Why should Product A1227, ABTS Enhancer, be used?

    1 answer
    1. This enhancer may be used to increase sensitivity or to conserve assay components.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. How can Product A1227, ABTS Enhancer, be used?

    1 answer
    1. This product is for microwell applications such as ELISA. A soluble reaction product is produced, so this is not for applications that require a precipitation reaction such as blotting or histochemical applications.

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