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A3219

Sigma-Aldrich

2,2'-叠氮-双（3-乙基苯并噻唑啉-6-磺酸）

peroxidase substrate, chromogenic, liquid

同義詞:

ABTS

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About This Item

MDL號碼:
MFCD00214141
分類程式碼代碼:
12352204
PubChem物質ID:
24890730
NACRES:
NA.83
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產品名稱

2,2'-叠氮-双（3-乙基苯并噻唑啉-6-磺酸）, Liquid Substrate System

品質等級

200

形狀

liquid

儲存溫度

2-8°C

SMILES 字串

CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O

InChI

1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-

InChI 密鑰

ZTOJFFHGPLIVKC-CLFAGFIQSA-N

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相關類別

一般說明

该产品作为过氧化物酶的即用型单组分底物提供，所述过氧化物酶在酸性缓冲液中由 2,2′-联氮-双（3-乙基苯并噻唑啉-6-磺酸）组成。底物与过氧化物酶反应前无色，与之反应后产生蓝绿色的显色反应产物。在终点检测期间，可使用 1% 十二烷基硫酸钠 (SDS) 终止反应。由于该底物会产生可溶性反应产物，因此不推荐用于免疫印迹或组织化学应用。

應用

2,2'-叠氮化合物-双（3-乙基苯并噻唑啉-6-磺酸）已被用作 ELISA 实验中的过氧化物酶底物。该底物得到绿色可溶性最终产物。[1][2]它也被用来作为一种胶体制剂散布在标准的培养基平板上，以评估木质素的氧化活性。底物呈绿色显示微生物漆酶活性。[3]

生化／生理作用

2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）(ABTS) 是一种阳离子，在过氧化氢存在下发生氧化。氧化 ABTS 为蓝绿色产品，可通过比色法测量。[4]ABTS、过氧化氢和肌红蛋白相结合可用作测量样本总抗氧化能力 (TAC) 的标准系统。[5]ABTS 方法在不同 pH 值下具有灵活性，并且应用最广泛。[6]ABTS 是酚氧化酶的底物。[7]

外觀

即用型。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCW8098
MKCV7001
MKCT8008
MKCW3025
MKCT0476

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存取文件庫

ABTS assay of phenol oxidase activity in soil
Floch C, et al.
Journal of Microbiological Methods, 71(3), 319-324 (2007)
Wei Xin et al.
Infection and immunity, 76(7), 3241-3254 (2008-05-07)
Recombinant attenuated Salmonella vaccines (RASVs) have been used extensively to express and deliver heterologous antigens to host mucosal tissues. Immune responses can be enhanced greatly when the antigen is secreted to the periplasm or extracellular compartment. The most common method
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
Erel O
Clinical Biochemistry, 37(4), 277-285 (2004)
Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
Shalaby EA and Shanab SMM
Indian Journal of Geo-Marine Sciences, 42(5), 556-564 (2013)
Randall L Davis et al.
Journal of neuroimmunology, 186(1-2), 141-149 (2007-05-04)
Emerging evidence indicates that neuroinflammatory responses in astroglia, including chemokine expression, are altered by opioids. Astroglial chemokines, such as CXCL10, are instrumental in response to many neuropathological insults. Opioid mediated disruption of astroglial CXCL10 expression may be detrimental in opioid
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文章

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

1–8 of 8 Questions  

  1. Can this product be used for antioxidant assay?

    1 answer

    1. This product has not been specifically tested for use in antioxidant assays. However, it has been used successfully as such in several publications. Please see the link below to review one example:
      https://www.sciencedirect.com/science/article/pii/S0009912003002315?via%3Dihub

      Helpful?

  2. What is the approximate concentration of H2O2 in the ready-to-use A3219 solution?

    1 answer

    1. This product is no longer tested for hydrogen peroxide concentration. The previous specification for H2O2 was </= 0.03%. Current lots are expected to be within this range.

      Helpful?

  3. What is the molecular weight and density?

    1 answer

    1. This product is a 1.8 mM solution of ABTS, the density is not determined. The solution is prepared from a diammonium salt form with a molecular weight of 548.60.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf

      Helpful?

  4. ABTS ready to use를 사용하여 항산화 실험을 하는경우 기존의 방법에 적용하면 어떤 단계가 생략이 되는건지 자세한 실험 과정을 알고 싶습니다

    1 answer

    1. The only protocol currently available on the website is this data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf.

      It may be helpful to also view the "Peer-Reviewed Papers" related to this product: https://www.sigmaaldrich.com/search/a3219?focus=papers&page=1&perpage=30&sort=relevance&term=a3219&type=citation_search.

      Helpful?

  5. When using Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at what wavelength should the product of the reaction be measured?

    1 answer

    1. The product should be measured at 405 nm. For end point assays, the reaction may be stopped with 1% (w/v) SDS and also read at 405 nm.

      Helpful?

  6. What is the concentration of Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)?

    1 answer

    1. The concentration of the ABTS is approximately 1.8 mM.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Should Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), be diluted prior to use?

    1 answer

    1. No, this product is offered as a ready-to-use (1×) solution.

      Helpful?

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