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重要文件

A1031

Sigma-Aldrich

α-淀粉酶 来源于人类唾液

greener alternative

Type XIII-A, lyophilized powder, 300-1,500 units/mg protein

同義詞:

1,4-α-D-葡聚糖-葡萄糖水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54
暫時無法取得訂價和供貨情況

種類

Type XIII-A

品質等級

形狀

lyophilized powder

比活性

300-1,500 units/mg protein

成份

Protein, 5-15% biuret

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

UniProt登錄號

環保替代類別

儲存溫度

−20°C

基因資訊

human ... AMY1A(276)

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一般說明

人α-淀粉酶由包含496个氨基酸的单个多肽链组成,其在1号染色体上进行编码。其在唾液腺或胰腺中产生。唾液和胰腺α-淀粉酶具有同源的一级序列,但表现出不同的切割模式。[1]
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时,该产品经优化可提高能效和防止浪费。有关详细信息,请参阅 《Biofiles》的文章

應用

α-淀粉酶可用于水解α-连接多糖(例如淀粉和糖原)的α键。产品A1031来自人唾液,属IIA型,并以冻干粉末形式提供。α-淀粉酶已被用于各种植物研究,如 拟南芥 的代谢研究 [2]。 来自人类唾液的α-淀粉酶已被用于营养保健品的开发,其可能有助于治疗糖尿病和肥胖症 [3]

生化/生理作用

α-淀粉酶可水解三个或更多个D-葡萄糖单元通过α-(1,4)连接的多糖中的α-(1,4)葡聚糖键。天然底物(如淀粉和糖原)可被分解成葡萄糖和麦芽糖。[4]

單位定義

在pH 6.9、温度20 °C条件下,一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

外觀

含有(NH4)2SO4、柠檬酸钠和氯化钠的冻干粉。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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存取文件庫

Lana Zupancic Cepic et al.
Oral diseases, 26(5), 1072-1080 (2020-03-04)
This study aimed to characterize surface properties such as roughness (Ra) and surface-free energy (SFE) of glazed and polished yttria-stabilized zirconia and to evaluate in vitro adherence of fungus Candida albicans and salivary bacteria, Staphylococcus epidermidis, mixed with C. albicans
Aleix Lluansí et al.
Frontiers in microbiology, 12, 716307-716307 (2021-10-29)
Inflammatory bowel disease (IBD), including its two main categories (Crohn's disease and ulcerative colitis), has been linked both to gut microbiota and to diet. Bread is a daily food that has a potential capacity as a prebiotic. Our aim was
Digestive enzyme inhibitors from grains as potential components of nutraceuticals.
D Piasecka-Kwiatkowska, et al.
J. Nutr. Sci. Vitaminol., 58, 217-220 (2012)
G D Brayer et al.
Protein science : a publication of the Protein Society, 4(9), 1730-1742 (1995-09-01)
The structure of human pancreatic alpha-amylase has been determined to 1.8 A resolution using X-ray diffraction techniques. This enzyme is found to be composed of three structural domains. The largest is Domain A (residues 1-99, 169-404), which forms a central
Camila Gabriel Kato-Schwartz et al.
Food research international (Ottawa, Ont.), 137, 109462-109462 (2020-11-26)
A practical approach to control glycemia in diabetes is to use plant natural products that delay hydrolysis of complex sugars and promote the diminution of the release of glucosyl units into the blood plasma. Polyphenolics have been described as being

條款

按照我們的程序來測定 alpha-Amylase 活性。此 a-Amylase 鹼性淀粉酶測法可引導您完成整個過程和必要的計算。

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Questions

1–4 of 4 Questions  
  1. Hi, could you let me know what the remaining composition is when the product contains 5-15% protein? Thank you!

    1 answer
    1. As mentioned in the 'DESCRIPTION' section, this product is a lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride. This product is not tested for purity. However, buffer salts are expected to be the bulk of the non-protein content.

      Helpful?

  2. Please let me know the storage temperature and storage period when it is dissolved in water

    1 answer
    1. The stability of this material in solution has not been investigated. However, various sources suggest that solutions may be stored in working aliquots at -20°C for several months.

      Helpful?

  3. What is the recommended method for dissolving alpha amylase obtained from human saliva, specifically A1031?

    1 answer
    1. The recommended method is to dissolve the enzyme in 1mM calcium chloride (CaCl2).

      Helpful?

  4. Could you please tell me the weight (mg or gram) of sample A1031?

    1 answer
    1. This product is sold based on units of activity. The activity specification is 300 - 1500 U/mg. The unit per milligram value is reported in the lot-specific Certificate of Analysis and will range from 0.67 to 3.3 mg for the 1000 U pack size.

      See the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A1031-1KU0000270112.pdf

      Helpful?

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