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P3803

Sigma-Aldrich

苯酚:氯仿:异戊醇25:24:1，10mM Tris饱和溶液，pH8.0，1mM EDTA

for molecular biology

同義詞:

DNA extraction

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52
等級:
for molecular biology
化驗:
≥99.0%
應用:
agriculture
暫時無法取得訂價和供貨情況

等級

for molecular biology

品質等級

200

化驗

≥99.0%

形狀

liquid

pH值

6.5-6.9(pH of phenolic phase)

應用

agriculture

儲存溫度

2-8°C

應用

使用苯酚:氯仿:异戊醇溶液提取，可以从核酸中去除蛋白质。[1][2][3][4]对于核酸粗提混合物，在使用苯酚:氯仿:异戊醇溶液提取前，最好先用一系列蛋白水解酶消解。尽管蛋白质能够被苯酚有效变性，核酸酶活性并未被完全抑制。因此需加入少量异戊醇，进一步确保抑制核酸酶活性。请查阅我们的产品信息表，获得从核酸中去除蛋白质的实验方案。

警告

如果苯酚接触到皮肤，会很快烧伤。因此，使用者需要穿着合适的实验服，包括手套和眼睛护具。如果直接接触到皮肤，立刻用大量水冲洗，并且寻求医学救助。

其他說明

该产品的保质期是两年。

推薦產品

进行DNA提取时，可以用Tris缓冲液平衡，将苯酚相的pH调整到8.0。Sigma提供专门用于DNA提取的P2069产品。该产品包括了平衡缓冲液，能够方便地调节pH值。

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCX1196
MKCV7671
MKCV3474
MKCV3475
MKCT9743

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存取文件庫

Philippe Lefrançois et al.
BMC genomics, 10, 37-37 (2009-01-23)
Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S. cerevisiae. Although ChIP-Seq in mammalian cell lines is replacing
Jeong-Kui Ku et al.
Annals of translational medicine, 8(6), 314-314 (2020-05-02)
The manufacturing of the demineralized dentin matrix (DDM) has been proven to extensively reduce the presence of human hepatitis B viral DNA (HBV DNA). This study measured and compared HBV DNA in fresh dentin to that in gamma radiation (GR)-sterilized
Tzemach Aouizerat et al.
Bio-protocol, 10(1), e3473-e3473 (2020-01-05)
Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a
Exogenous controls increase negative call veracity in multiplexed, quantitative PCR assays for Phakopsora pachyrhizi
James Haudenshield
Plant Disease, 95.3, 343-352 (2011)
Bizunesh Abere et al.
mBio, 11(6) (2020-12-17)
Viral noncoding RNAs have acquired increasing prominence as important regulators of infection and mediators of pathogenesis. Circular RNAs (circRNAs) generated by backsplicing events have been identified in several oncogenic human DNA viruses. Here, we show that Merkel cell polyomavirus (MCV)
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Questions

1–3 of 3 Questions  

  1. I received two bottles. One for the phenol and one for the buffer. Do I need to add the phenol to the buffer? or the buffer already contains the phenol?

    1 answer

    1. Yes, the equilibration buffer is to be added before use, according to the following instructions:
      Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol reagent.
      Mix gently and allow the phases to separate before use, typically 2–4 hours.
      This will adjust the pH of the phenol phase from pH 6.7±0.2 to 8.0±0.2.

      Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/490/315/p3803pis.pdf

      Helpful?

  2. I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?

    1 answer

    1. In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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