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重要文件

77618

Sigma-Aldrich

苯酚-氯仿-异戊醇混合物

Phenol – chloroform – isoamyl alcohol mixture
1 of 1 reviewers received a sample product or took part in a promotion

BioUltra, for molecular biology, 49.5:49.5:1

同義詞:

phenol:Chloroform:Isoamyl Alcohol

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352401
PubChem物質ID:
NACRES:
NA.07
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等級

Molecular Biology
for molecular biology

品質等級

產品線

BioUltra

形狀

liquid

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值

7.7-8.3(H2O-phase, after extraction with H2O, 1:1)

密度

1.29 g/mL at 20 °C

吸收

cut-off at 360 nm

儲存溫度

2-8°C

SMILES 字串

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI 密鑰

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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應用


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).

外觀

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

其他說明

Large- and small-scale phenol extractions[1]

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

177.8 °F - closed cup

閃點(°C)

81 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Large- and small-scale phenol extractions.
D M Wallace
Methods in enzymology, 152, 33-41 (1987-01-01)
Lei Wang et al.
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Estrogen and estrogen receptor (ER)-regulated gene transcriptional events have been well known to be involved in ER-positive breast carcinogenesis. Meanwhile, circular RNAs (circRNAs) are emerging as a new family of functional non-coding RNAs (ncRNAs) with implications in a variety of
Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the

相關內容

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Questions

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Review 1
    • Votes 0
    1 out of 5 stars.

    Phenol-Chloroform degraded

    The Phenol-Chloroform degraded. We called your company and has not receive any replacement yet..

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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