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Merck
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P1944

Sigma-Aldrich

苯酚/氯仿/异戊醇（125:24:1）

for molecular biology

同義詞:

phenol chloroform RNA extraction

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52

等級

for molecular biology

化驗

≥99.0%

形狀

liquid

pH值

4.3-4.7

應用

life science and biopharma

儲存溫度

2-8°C

一般說明

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

應用

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

標靶器官

Liver,Kidney, Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

175.0 °F - closed cup

閃點（°C）

79.44 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

分析證明 (COA)

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Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
Exploring translation regulation by global analysis of ribosomal association
Melamed D, et al.
Methods in Enzymology, 48(3), 301-305 (2009)
Molecular approaches for analysis of Drosophila MicroRNAs
Pandey M, et al.
Journal of Separation Science, 169-188 (2020)
Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds
Berardino JD, et al.
Journal of Experimental Botany, 69(6), 1403-1414 (2018)
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Ethidium Bromide Removal

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol from Cold Spring Harbor Laboratory Press.

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