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14533

Sigma-Aldrich

双苯并咪唑 H 33342 三盐酸盐

for fluorescence, ≥97.0% (HPLC)

同義詞:

2′-(4-乙氧基苯基)-5-(4-甲基-1-哌嗪基)- 2,5′-双-1H-苯并咪唑 三盐酸盐, HOE 33342, Hoechst 33342, 双苯并咪唑

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About This Item

經驗公式（希爾表示法）:
C27H28N6O · 3HCl · xH2O
CAS號碼:
875756-97-1
分子量：:
561.93 (anhydrous basis)
Beilstein:
1234011
EC號碼:
245-691-1
MDL號碼:
MFCD00012678
分類程式碼代碼:
12161505
PubChem物質ID:
57647173
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

for fluorescence

化驗

≥97.0% (HPLC)

螢光

λex 340 nm; λem 510 nm
λex 355 nm; λem 465 nm in TE buffer; DNA

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6

InChI

1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H

InChI 密鑰

FYEVKHPLBHLWHK-UHFFFAOYSA-N

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應用

bisBenzimide H 33342 三盐酸盐可用于研究生物化学和试剂、荧光探针、标记、颗粒和污渍、发光化合物和检测和波长指数。bisBenzimide H 33342 三盐酸盐，Hoechst 33342，已用于研究无机和有机砷化合物对人类 T 淋巴母细胞白血病细胞的影响。Hoechst 33342 也被用于研究 9 种钙拮抗剂在 HeLa 和 SN-38 抗性 HeLa（HeLa/SN100）细胞（过量表达 ABCG2/BCRP）中，对 ABCG2/BCRP 介导的抗性和转运的影响。
适用于DNA，染色体和细胞核染色。可用于荧光显微镜或流式细胞术。
最大激发波=346nm
最大发射波长=460nm

生化／生理作用

膜透性，具有低细胞毒性荧光DNA染料，插入在DNA的A-T区域。

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

其他說明

适用于各种细胞类型的重要 DNA染色[1][2]；作为哺乳动物细胞膜通透性的探针[3]

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
BCCN0628
BCCL3465
BCCJ9222
BCCH0905
BCCG8558

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M J Lydon et al.
Journal of cellular physiology, 102(2), 175-181 (1980-02-01)
A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is
W M Grogan et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(6), 738-746 (1981-06-01)
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells
M E Lalande et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(1), 363-367 (1981-01-01)
Flow cytometric analysis of the uptake of the DNA-specific and fluorescent probe Hoechst 33342 (HO342) offers a simple and rapid method for measuring membrane transport rates in mammalian cells and identifying cellular subpopulations that differ in their membrane transport rates.
Steven S Welc et al.
Nature communications, 10(1), 2788-2788 (2019-06-28)
Many potentially therapeutic molecules have been identified for treating Duchenne muscular dystrophy. However, targeting those molecules only to sites of active pathology is an obstacle to their clinical use. Because dystrophic muscles become extensively inflamed, we tested whether expressing a therapeutic
Fang-Hsin Chen et al.
International journal of radiation oncology, biology, physics, 86(4), 777-784 (2013-04-23)
To investigate vascular responses during fractionated radiation therapy (F-RT) and the effects of targeting pericytes or bone marrow-derived cells (BMDCs) on the efficacy of F-RT. Murine prostate TRAMP-C1 tumors were grown in control mice or mice transplanted with green fluorescent
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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. When Product 14533, bisBenzimide H 33342 trihydrochloride, is dissolved in water, what is the pH?

    1 answer

    1. The pH of a 2% solution in water is approximately 1.9.

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  5. What is the solubility of Product 14533, bisBenzimide H 33342 trihydrochloride?

    1 answer

    1. It is soluble in water at 50 mg/mL.  It will precipitate from phosphate buffer solutions

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