跳轉至內容
Merck
全部照片(1)

重要文件

檢視所有文件

03449

Sigma-Aldrich

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺 盐酸盐

≥99.0% (AT)

同義詞:

N-乙基-N′ -（3-二甲基氨基丙基）碳二亚胺 盐酸盐, EDAC, EDC, EDC 盐酸盐, WSC 盐酸盐

登入查看組織和合約定價
全部照片(1)

About This Item

經驗公式（希爾表示法）:
C8H17N3 · HCl
CAS號碼:
25952-53-8
分子量：:
191.70
Beilstein:
5764110
EC號碼:
247-361-2
MDL號碼:
MFCD00012503
分類程式碼代碼:
12161502
PubChem物質ID:
329747707
NACRES:
NA.21
暫時無法取得訂價和供貨情況

化驗

≥99.0% (AT)

形狀

powder

技術

bioconjugation: suitable

mp

110-115 °C (lit.)
112-116 °C

溶解度

H2O: soluble 0.2 g/L

儲存溫度

−20°C

SMILES 字串

Cl.CCN=C=NCCCN(C)C
Cl.CCN=C=NCCCN(C)C

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

InChI 密鑰

FPQQSJJWHUJYPU-UHFFFAOYSA-N

尋找類似的產品？ 前往 產品比較指南

一般說明

1-乙基-3-(3-二甲氨基丙基)碳二亚胺盐酸盐，通常称EDAC HCl，是化学和生化研究中广泛使用的一种有效的水溶性试剂，主要用于促进酰胺键的形成。EDAC HCl在肽合成中特别有效，它通过羧基和胺基偶联氨基酸，对于创建具有特定序列和功能的肽至关重要。EDAC HCl的影响不局限于多肽，它通过共价附着半抗原小分子来促进免疫原的构建，从而引发对载体蛋白的免疫反应，在疫苗研究中发挥关键作用。EDAC HCl的多功能性还扩展到核酸修饰，允许通过其5个磷酸基团标记DNA和RNA。这种能力促进了这些关键分子的可视化、跟踪和分析，为核酸研究的进步做出了重大贡献。

此外，EDAC HCl作为一个生物分子桥，充当交联剂连接生物分子的胺反应性NHS酯和羧基。这一技术在蛋白质偶联中被证明是无价的，能够创建具有不同性质和功能的杂交分子。潜在的机制涉及EDAC hcl与羧基的反应，形成一个不稳定的中间体，积极寻找胺伴侣。这种反应的微妙平衡强调了优化条件以确保有效共轭的重要性。N-羟基琥珀酰亚胺(NHS)的辅助通过稳定中间体和实现两步偶联程序进一步增强 EDAC HCl的能力。此额外功能提供了更大的灵活性和可控性，特别是在处理复杂的生物分子时。

應用

N-(二甲基氨基丙基)-N′-乙基碳二亚胺盐酸盐≥99.0% (At)已被用作共价固定酪氨酸酶、制备酶电极的活化剂。[1]此外，它还被用作组胺免疫标记的预固定剂。[2]
在锚定的双层脂质膜自组装过程中，N-（3-二甲基氨基丙基）-N′-乙基碳二亚胺盐酸盐已应用于胺偶联反应。[3]其还用于将抗COX5b抗体衍生化为羧化聚苯乙烯微球。[4]

生化／生理作用

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺是一种水溶性缩合剂。EDAC通常用作与伯胺进行酰胺键合的羧基活化剂。[5]另外，它能与磷酸基团反应。其已用于肽合成、将蛋白质与核酸交联、以及免疫缀合物的制备。[6]
水溶性缩合试剂。EDAC通常用作与伯胺进行酰胺键合的羧基活化剂。另外，它能与磷酸基团反应。其已用于例如肽合成、将蛋白质与核酸交联、以及免疫缀合物的制备。通常，EDAC在不含缓冲剂的pH范围4.0-6.0的条件下使用。特别是，应避免使用胺和羧酸盐缓冲液。

特點和優勢

用途广泛，适应各种实验室和研究应用

其他說明

如需了解生化试剂系列的更多信息，请填写此表。
水溶性肽偶联剂;[7] 修饰蛋白质中的羧基，综述;[8] 酯的合成。[9]

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

標靶器官

Stomach,large intestine,lymph node

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
BCCM9954
BCCM8000
BCCL6303
BCCM5330
BCCL9735

未看到正確版本？

如果您需要一個特定的版本，您可以透過批號來尋找特定憑證。

搜尋 COA

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Synthesis of esters.
Dhaon, M.K.
The Journal of Organic Chemistry, 47, 1962-1962 (1982)
Total synthesis of a monocyclic peptide lactone antibiotic, etamycin.
J C Sheehan et al.
Journal of the American Chemical Society, 95(3), 875-879 (1973-02-07)
A flight sensory-motor to olfactory processing circuit in the moth Manduca sexta
Bradley SP, et al.
Frontiers in Neural Circuits, 10(6), 5-5 (2016)
Xuchen Hu et al.
eLife, 8 (2019-09-06)
Cultured mouse peritoneal macrophages release large numbers of ~30-nm cholesterol-rich particles. Here, we show that those particles represent fragments of the plasma membrane that are pulled away and left behind during the projection and retraction of filopodia and lamellipodia. Consistent
Bing Wang et al.
mBio, 12(3), e0142321-e0142321 (2021-06-23)
The catalytic subunit of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) Nsp12 has a unique nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain that transfers nucleoside monophosphates to the Nsp9 protein and the nascent RNA. The NiRAN and
查看所有相關論文

文章

用於生物和化學傳感器的石墨烯場效電晶體

生物標誌物的檢測和量化對於醫療診斷、環境監控和生物研究是非常重要的。

Graphene Field Effect Transistors for Biological and Chemical Sensors
Functionalized Graphene Oxide: Properties & Applications

Professor Aran discusses engineering graphene-based materials through careful functionalization, enabling diverse applications.

相關內容

Properties and Applications of Functionalized Graphene Oxide

Professor Aran (Claremont University, USA) thoroughly discusses the engineering of graphene based materials through careful functionalization of graphene oxide, a solution processable form of graphene.

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務