Activation is not required for this preparation. However, the beads should be washed thoroughly prior to use y with 5-10 column volumes of buffer, to remove the sodium azide in the storage buffer. Please see the link below to review the datasheet for product S1638, this may be applied to this material.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/273/s1638pis-ms.pdf
推薦產品
形狀
suspension
品質等級
包含
0.02% NaN3 as preservative
技術
affinity chromatography: suitable
容量
≥15 μg/mL, packed gel capacity (biotin)
儲存溫度
2-8°C
一般說明
通过 C6-间隔臂连接到 4% 微粒交联琼脂糖上的链霉抗生物素蛋白(1.2mg 链霉抗生物素蛋白/mL 填充凝胶)
外觀
50% 悬浮液溶于 0.01M 磷酸钠缓冲液中,pH 7.2,含 0.15M 氯化钠
其他說明
通过生物素化刀豆蛋白A和固定化链霉亲和素蛋白分离细胞表面糖蛋白[1]。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves
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I want to know if before using the beads I need activate it with any specific solution. Could you help me please.
1 answer-
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