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85881

Supelco

链霉抗生物素蛋白,固定在琼脂糖 CL-4B 上

suitable for affinity chromatography

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.21
暫時無法取得訂價和供貨情況

形狀

suspension

品質等級

包含

0.02% NaN3 as preservative

技術

affinity chromatography: suitable

容量

≥15 μg/mL, packed gel capacity (biotin)

儲存溫度

2-8°C

一般說明

通过 C6-间隔臂连接到 4% 微粒交联琼脂糖上的链霉抗生物素蛋白(1.2mg 链霉抗生物素蛋白/mL 填充凝胶)

外觀

50% 悬浮液溶于 0.01M 磷酸钠缓冲液中,pH 7.2,含 0.15M 氯化钠

其他說明

通过生物素化刀豆蛋白A和固定化链霉亲和素蛋白分离细胞表面糖蛋白[1]

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Jilian Su et al.
Molecular and cellular probes, 46, 101415-101415 (2019-06-23)
Vascular endothelial growth factor (VEGF) can promote cell division, proliferation and migration. In this study, we aimed to investigate roles of ultrasound-mediated destruction of VEGF-targeted and paclitaxel (PTX)-loaded lipid microbubbles (VTPLLM + US) in human breast cancer cells. The activity of MCF-7 cells
J W Buckie et al.
Analytical biochemistry, 156(2), 463-472 (1986-08-01)
An indirect affinity chromatography procedure utilizing biotinylated lectins and designed for the specific isolation of surface glycoproteins is described. The method is illustrated with intact acute leukemic lymphoblastic cells (ALL cells) with biotin-epsilon-aminocaproyl-concanavalin A (biocap-Con A) and streptavidin-Sepharose 4B. Biocap-Con
Yahui Huang et al.
Clinical & experimental metastasis, 37(4), 477-487 (2020-07-03)
MACC1 (metastasis associated in colon cancer 1) is a key driver that induces metastasis in colon cancer. However, the mechanisms by which MACC1 expression is transcriptionally regulated and the factors enriched at the MACC1 promoter remain largely unknown. The binding
Romina B Cejas et al.
Biological chemistry, 401(9), 1041-1051 (2020-04-17)
Glycosylation is a very frequent post-translational modification in proteins, and the initiation of O-N-acetylgalactosamine (O-GalNAc) glycosylation has been recently described on relevant nuclear proteins. Here we evaluated the nuclear incorporation of a second sugar residue in the biosynthesis pathway of
Yen-Jung Chen et al.
Antioxidants & redox signaling, 31(2), 109-126 (2018-11-13)
Aims: The coordination of neurons to execute brain functions requires plenty of oxygen. Thus, it is not surprising that

Questions

  1. I want to know if before using the beads I need activate it with any specific solution. Could you help me please. 

    1 answer
    1. Activation is not required for this preparation. However, the beads should be washed thoroughly prior to use y with 5-10 column volumes of buffer, to remove the sodium azide in the storage buffer. Please see the link below to review the datasheet for product S1638, this may be applied to this material.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/273/s1638pis-ms.pdf

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