This product contains lithium dodecyl sulfate with pH at 8.4, but does not include a reducing agent. Either Dithiothrietol (DDT) or beta-mercaptoethanol (BME) may be used. The recommended final concentration is 100 mM DTT or 25 mM BME. Please see the link below to access the mPAGE™ Bis-Tris Precast SDS-PAGE Gels User Guide, page 7:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/384/513/20344106w-ms.pdf
推薦產品
形狀
liquid
包裝
vial of 10 mL
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相關類別
一般說明
用于制备变性聚丙烯酰胺凝胶电泳(PAGE)的蛋白样品，适合mPAGE凝胶和其他大多数Bis-Tris凝胶。LDS样品缓冲液含有十二烷基硫酸锂，pH 8.4，适合还原剂保持最佳活性。
- 除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
應用
蛋白质印迹
法律資訊
MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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What is the composition of the loading buffer. Does it contain any reducing agents?
1 answer-
Helpful?
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Can Laemmli buffer be used instead of the sample buffer?
1 answer-
Laemmli buffer is commonly used with Tris-Glycine gels, while mPAGE gels use a different chemistry (Bis-Tris) and require LDS buffer. Therefore, it is recommended to use the LDS buffer, which can be prepared using the formula provided in the mPAGE user guide.
Helpful?
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Est ce que ce tampon contient déjà un agent réducteur tel que le DTT ou le béta mercapto ethanol ?
1 answer-
This product requires the addition of a reducing agent. Either Dithiothrietol (DDT) or beta-mercaptoethanol (BME) may be used. The recommended final concentration is 100 mM DTT or 25 mM BME. Please see the link below to access the mPAGE™ Bis-Tris Precast SDS-PAGE Gels User Guide, page 7:
https://www.emdmillipore.com/US/en/product/mPAGE-8-160-0-Bis-Tris-Precast-Gel-10x8-cm-12-well,MM_NF-MP81G12#documentationHelpful?
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