推薦產品
應用
样品缓冲液,Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。
生化/生理作用
Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液(1:1)混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性,并给每个蛋白质和亚基带来整体的负电荷,以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料,也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。
其他說明
含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。
數量
推荐使用方法:将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。
相關產品
產品號碼
描述
訂價
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
儲存類別代碼
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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條款
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
蛋白質抗原分子量測定、蛋白質互作、酵素活性和轉譯後修飾的技術。
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
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