跳轉至內容
Merck
全部照片(1)

文件

S3401

Sigma-Aldrich

样品缓冲液,Laemmli 2x 浓缩液

同義詞:

SDS样品缓冲液

登入查看組織和合約定價


About This Item

分類程式碼代碼:
41105323
NACRES:
NA.25

應用

样品缓冲液,Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。

生化/生理作用

Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液(1:1)混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性,并给每个蛋白质和亚基带来整体的负电荷,以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料,也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。

其他說明

含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。

數量

推荐使用方法:将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

儲存類別代碼

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

客戶也查看了

Slide 1 of 1

1 of 1

GADD45A and EPB41 as tumor suppressor genes in meningioma pathogenesis.
Piaskowski S, et al.
Cancer Genetics and Cytogenetics, 162, 63-67 (2005)
Dana E Nuti et al.
mBio, 2(4), doi:10-doi:10 (2011-08-19)
Detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a
Amanda M Gonzales et al.
Nutrition & metabolism, 5, 17-17 (2008-06-14)
Adipocytes express inflammatory mediators that contribute to the low-level, chronic inflammation found in obese subjects and have been linked to the onset of cardiovascular disorders and insulin resistance associated with type 2 diabetes mellitus. A reduction in inflammatory gene expression
Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
Sidhu H, et al.
The Journal of Neuroscience, 34, 9867-9879 (2014)
Maria Marín et al.
Genes, 10(1) (2019-01-20)
XPF endonuclease is one of the most important DNA repair proteins. Encoded by XPF/ERCC4, XPF provides the enzymatic activity of XPF-ERCC1 heterodimer, an endonuclease that incises at the 5' side of various DNA lesions. XPF is essential for nucleotide excision

條款

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

蛋白質抗原分子量測定、蛋白質互作、酵素活性和轉譯後修飾的技術。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務