跳轉至內容
Merck
全部照片(7)

重要文件

檢視所有文件

MAB4401

Sigma-Aldrich

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon®, from mouse

同義詞:

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

登入查看組織和合約定價
全部照片(7)

About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
暫時無法取得訂價和供貨情況

生物源

mouse

抗體表格

purified antibody

無性繁殖

10H11.2, monoclonal

物種活性

human

製造商／商標名

Chemicon®

技術

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

輸入

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

同型

IgG1

NCBI登錄號

NM_002701.4

UniProt登錄號

Q01860

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... POU5F1(5460)

一般說明

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Purified monoclonal antibody for the detection of Human Oct-4.

特異性

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

應用

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

品質

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

標靶描述

44 kDa

外觀

Format: Purified

分析報告

Control
Human embryonic stem cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
Methods in Cell Biology null
Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
Cellular Reprogramming null
Li Zou et al.
Scientific reports, 6, 22868-22868 (2016-03-17)
Human pluripotent stem cells provide a standardized resource for bone repair. However, criteria to determine which exogenous cells best heal orthopedic injuries remain poorly defined. We evaluated osteogenic progenitor cells derived from both human embryonic stem cells (hESCs) and induced
Lingjun Rao et al.
Nature communications, 9(1), 126-126 (2018-01-11)
The generation of functional skeletal muscle tissues from human pluripotent stem cells (hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) via transient overexpression of Pax7 in paraxial mesoderm cells differentiated from hPSCs. In 2D culture
Chen Liu et al.
PloS one, 9(9), e108239-e108239 (2014-09-27)
Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels.
查看所有相關論文

文章

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

無血清、無飼料的定義培養基，可實現無週期培養未分化的人類 ES/iPS 細胞

跳過週末餵食。人類多能幹細胞（ES 和 iPS 細胞）的定義無血清和無飼料擴展培養基。請參閱刊物和協議。

HumanKine® Thermostable bFGF

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

查看全部

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務