MAB4419

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

clone 7F9.2, from mouse

• 同義詞: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 7F9.2, monoclonal
• 物種活性: human, mouse
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• 輸入: sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)
• 同型: IgG1κ
• NCBI登錄號: NP_976034
• UniProt登錄號: P20263
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... POU5F1(5460) ; mouse ... Pou5F1(18999)

描述

一般說明

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

特異性

Reactivity to other species has not been determined.

This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

免疫原

Recombinant human Oct-4 protein, a.a. 1-145.

應用

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].

Western Blotting: Recommended working dilution is 1 - 2 µg/mL.

Flow Cytometry: Recommended working dilution is 2 µg/mL.

Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.

標靶描述

~39 kDa

聯結

Replaces: MAB4305

外觀

Protein G sepharose column

Format: Purified

Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.

儲存和穩定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable