This product does not require activation. Note that this material has not been tested for use in cell culture. Suitability would have to be determined by the end user.
推薦產品
生物源
human
品質等級
化驗
>98% (SDS-PAGE)
形狀
solid
製造商／商標名
Chemicon®
技術
activity assay: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
基因資訊
human ... PLAU(5328)
一般說明
产品来源：由健康人的尿液制备
将纤溶酶原转化为纤溶酶的溶栓剂。 高分子量和低分子量尿激酶的混合物。
外觀
从 200 mM 甘露醇、100 mM NaCl、50 mM Tris-HCl、pH 7.5 和 0.1 % PEG 中冻干。
儲存和穩定性
使用 200-400 μL dH2O 复溶。储存于 -20°C。 溶解后，等分并冷冻保存（-70°C）。 本产品供货后可稳定保存 2 年。 储备液可在-70°C最长稳定6个月。
分析報告
高分子量尿激酶 (（54 kD）：40%。低分子量尿激酶（33 kD）：50%。尿激酶的暂时性形式：10%
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Thrombolytic therapy: a comparison between urokinase and streptokinase. From a national cooperative study.
W R Bell
Seminars in thrombosis and hemostasis, 2(1), 1-13 (1975-07-01)
Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968.
Johnson, A J, et al.
Thrombosis et Diathesis Haemorrhagica, 21, 259-272 (1969)
Sean T Zuckerman et al.
Biomaterials, 30(23-24), 3825-3833 (2009-05-16)
Mass spectrometry is a powerful proteomic tool enabling researchers to survey the global proteome of a cell. This technique has only recently been employed to investigate cell-material interactions. We had previously identified material scarcity and limited adherent cells as challenges
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Hello, I am interested in adding uPA to the medium of my cell culture of human pluripotent stem cell. Is product number CC4000 suitabl and ready to add to the medium, or does the protein needs activation before usage?
1 answer-
Helpful?
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Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
1 answer-
This product is a combination of active (low molecular weight) and inactive (high molecular weight) urokinase. The composition of the CC4000 product is as follows:
Low molecular weight urokinase (33 kD) 50%
High molecular weight urokinase (54 kD) 40%
Transient forms of urokinase 10%
Activation prior to use is not required.
uPA is produced as an inactive form called pro-uPA which is 54KDa. Pro-uPA is cleaved by proteases to produce a low molecular weight active form that is 33 kDa. This cleavage by plasmin occurs in two steps. The transitory form is the result of the first cleavage step. The second cleavage of this transient form results in the active 33 kDa uPA. The link below may provide additional helpful information:Helpful?
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