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CBA415

Sigma-Aldrich

用于3D和2D细胞培养基的活死细胞活力测定试剂盒

Live-dead cell viability kit for 3D and 2D cell cultures. Indicates live, dead and total cell count. Sufficient for 5 x 24-well plates or 12 x 96-well plates.

同義詞:

Viability Assay Kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84
暫時無法取得訂價和供貨情況

檢測方法

fluorometric

一般說明

活死细胞活力测定试剂盒是一种快速简便的三色测定方法，用于测量细胞活力。该试剂盒由Calcein-AM（活细胞染色）、碘化丙（死细胞染色）和Hoechst 33342（所有细胞染色）组成。该试剂盒已优化为3D细胞培养（球体、人体类器官和3D基质）和2D细胞培养（多种细胞类型）。该试剂盒可用于流式细胞术，荧光显微镜和荧光微孔板阅读器。该试剂盒根据细胞膜完整性和酯酶活性测定细胞活力。

一个试剂盒包含的试剂足够5 × 24孔板或12 × 96孔板使用。

<bold>优势：</bold>
1.针对3D细胞培养、球形细胞培养、类器官培养、2D细胞培养和流式细胞术进行了测试和优化。
2.易于使用和快速协议。
3.与其他产品相比，Calcein AM和Hoechst染料更强。

光谱属性
Calcein AM：Ex/Em：490/515 nm（绿色）
碘化丙啶：Ex/Em：535/617 nm（红色）
Hoechst 33342：Ex/Em：361/486 nm（蓝色）

协议：
2D单层细胞培养、3D细胞培养、3D球体和3D类器官的染色方案包括在数据表中。

儲存和穩定性

在-20oC或更低环境中避光保存。小瓶可以冷冻和解冻多达10次。使用前，让小瓶解冻至室温并短暂离心。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

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Questions

1–3 of 3 Questions  

  1. Is it possible to use formaldehyde or formalin for fixing cells before utilizing this live/dead cell staining solution?

    1 answer

    1. The use of this kit on formaldehyde or formalin fixed cells has not been assessed in our lab, and our literature survey has not provided a recommended protocol reference.

      It's important to note that fixing cells with a crosslinker such as formalin or formaldehyde could potentially impact the results of this assay. The harsh conditions of crosslinking may lead to cell membrane damage and increased cell death. As this has not been tested in our lab, we highly recommend conducting a pilot experiment to confirm compatibility.

      Helpful?

  2. Could this product be used for live imaging for 72hours ?

    1 answer

    1. Working solutions of the Propidium Iodide and the Hoechst 33342 are stable for at least 72 hours when refrigerated and protected from light. Calcein AM, once diluted to a working concentration in a aqueous diluent, will begin to degrade within several hours and storage is not recommended.

      Helpful?

  3. How long can I keep the dye solution for after I add the dyes in the PBS and media? 

    1 answer

    1. The dye solution for cell staining should be prepared just before use. Store at -20oC or below, and protect from light. The vials can be frozen and thawed up to 10 times. Before use, allow the vials to thaw to room temperature and centrifuge briefly.

      Helpful?

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