529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem^®

• 同義詞: Pro-survival Compound, DDD00033325, DDD00033325
• 經驗公式（希爾表示法）: C₁₇H₁₉N₅
• CAS號碼: 1259034-82-6
• 分子量：: 293.37
• 分類程式碼代碼: 51111800
• NACRES: NA.77

屬性

• 產品名稱: Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.
• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: syrup
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: yellow
• 溶解度: DMSO: 100 mg/mL
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• SMILES 字串: C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2
• InChI: 1S/C17H19N5/c1-2-4-14(5-3-1)12-21-10-7-15(13-21)19-16-8-11-22-17(20-16)6-9-18-22/h1-6,8-9,11,15H,7,10,12-13H2,(H,19,20)/t15-/m1/s1
• InChI 密鑰: ZJVYTXWKNDTQNC-OAHLLOKOSA-N

描述

一般說明

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

包裝

Packaged under inert gas

警告

Toxicity: Regulatory Review (Z)

重構

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

其他說明

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable