推薦產品
化驗
>99% (TLC)
形狀
liquid
包裝
pkg of 1 × 1 mL (810601C-1mg)
製造商／商標名
Avanti Research™ - A Croda Brand 810601C
濃度
1 mg/mL (810601C-1mg)
脂質類型
ESR probes
phospholipids
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins[1] and as fluorescent quenchers in lipid bilayer structural studies.[2]
Phosphatidylcholine (PC), a strong bilayer-forming lipid is the most common phospholipid in mammalian membranes.[3] It is also an important component of the mucosal layer of the colon.[4] The 5th carbon of the sn-2 stearic acid chain of 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine analog has a Doxyl PC, a spin probe[5] attached to it covalently.[6]
應用
16:0-5 Doxyl PC may be used:
- as a component in virus-like large unilamellar vesicles (VL LUVs) to quench 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence emission[7]
- in the preparation of multi-lamellar vesicles (MLVs) as a site-specific quencher to perform fluorescence quenching studies[8]
- in the preparation of spin-labelled multi-lamellar vesicles (MLVs)[9]
生化／生理作用
包裝
5 mL Clear Glass Sealed Ampule (810601C-1mg)
準備報告
To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies.[11] For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM.[2], Supplemental information
法律資訊
Avanti Research is a trademark of Avanti Polar Lipids, LLC
訊號詞
Danger
危險分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
標靶器官
Central nervous system, Liver,Kidney
水污染物質分類（WGK）
WGK 3
The Membranes of Cells (2016)
Integrative Medicine - E-Book (2017)
Edurne Rujas et al.
The Journal of biological chemistry, 292(13), 5571-5583 (2017-02-19)
The 4E10 antibody displays an extreme breadth of HIV-1 neutralization and therefore constitutes a suitable model system for structure-guided vaccine design and immunotherapeutics against AIDS. In this regard, the relevance of autoreactivity with membrane lipids for the biological function of
Local polarity and hydrogen bonding inside the Sec14p phospholipid-binding cavity: high-field multi-frequency electron paramagnetic resonance studies.
Smirnova TI, et al.
Biophysical Journal, 92, 3686-3695 (2007)
Fayi Wu et al.
Biochemistry, 45(41), 12510-12518 (2006-10-13)
The putative substrate-binding site in lipoxygenases is long and internal. There is little direct evidence about how the unsaturated fatty acid substrates enter and move within the cavity to position themselves correctly for electron transfer reactions with the catalytic non-heme
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