推薦產品
化驗
>99% (TLC)
形狀
powder
包裝
pkg of 1 × 1 mg (810601P-1mg)
製造商／商標名
Avanti Research™ - A Croda Brand 810601P
脂質類型
ESR probes
phospholipids
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.[1][2]
Phosphatidylcholine (PC), a strong bilayer-forming lipid is the most common phospholipid in mammalian membranes.[3] The excretory and secretory products of helminths has a small hapten-like portion called phosphorylcholine (PC).[4] The 5th carbon of the sn-2 stearic acid chain of 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine analog has a Doxyl PC, a spin probe attached to it covalently.[5]
應用
16:0-5 Doxyl PC may be used:
- as a component in virus-like large unilamellar vesicles (VL LUVs) to quench 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence emission[6]
- in the preparation of multi-lamellar vesicles (MLVs) as a site-specific quencher to perform fluorescence quenching studies[7]
- in the preparation of spin-labelled multi-lamellar vesicles (MLVs)[8]
生化／生理作用
Phosphatidylcholine (PC) lowers the levels of cholesterol and triglycerides.[9]
包裝
5 mL Clear Glass Sealed Ampule (810601P-1mg)
準備報告
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies.[10] For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM [1], Additional supplemental information.
法律資訊
Avanti Research is a trademark of Avanti Polar Lipids, LLC
儲存類別代碼
11 - Combustible Solids
The Membranes of Cells (2016)
Host defenses to helminths
Clinical Immunology, 367-377 (2013)
Diego E Sastre et al.
The Journal of biological chemistry, 295(7), 2136-2147 (2019-12-05)
PlsX plays a central role in the coordination of fatty acid and phospholipid biosynthesis in Gram-positive bacteria. PlsX is a peripheral membrane acyltransferase that catalyzes the conversion of acyl-ACP to acyl-phosphate, which is in turn utilized by the polytopic membrane
Fayi Wu et al.
Biochemistry, 45(41), 12510-12518 (2006-10-13)
The putative substrate-binding site in lipoxygenases is long and internal. There is little direct evidence about how the unsaturated fatty acid substrates enter and move within the cavity to position themselves correctly for electron transfer reactions with the catalytic non-heme
H C Chan et al.
Magnetic resonance in medicine, 8(2), 160-170 (1988-10-01)
The positively charged nitroxide spin label, 2,2,6,6-tetramethyl-piperidine-N-oxyl-4-trimethylammonium (Cat1), was encapsulated in two types of liposomes, phosphatidylserine/phosphatidylcholine (PS/PC) and phosphatidylserine/distearoylphosphatidylcholine/dipalmitoylphosphatidyl choline (PS/DSPC/DPPC). The liposomes were incubated with mouse thymus-bone marrow (TB) cells to study the uptake and metabolism of nitroxides entrapped
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