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Merck
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SHC003V

Sigma-Aldrich

MISSION®

Green fluorescent protein marker to monitor transduction efficiency

同義詞:

MISSION®, MISSION® TurboGFP™ Control Transduction Particles

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About This Item

分類程式碼代碼:
41106609
NACRES:
NA.51

品質等級

100
200

產品線

MISSION®

濃度

≥1x106 VP/ml (via p24 assay)

技術

capture ELISA: 106 TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

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相關類別

一般說明

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP™ transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP™ (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

應用

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

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SHC002

任务 ® pLKO.1-puro 非哺乳动物 shRNA 对照质粒 DNA, Targets no known mammalian genes

SHC001V

MISSION®pLKO.1-puro空载体对照转导颗粒, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ 阳性对照质粒 DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION®, Targets no known mammalian genes

SHC004

MISSION® 对照载体, shRNA sequence targeting tGFP

SHC004V

MISSION® TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012V

MISSION®, Contains a gene encoding TagRFP

SHC014V

MISSION®, Contains a gene encoding TurboGFP, under the control of the UbC promoter

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

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Garvey CM
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We previously determined that cancer-stromal interaction was a direct route to tumor cell heterogeneity progression, since cancer-stromal cell fusion in coculture resulted in the creation of heterogeneous clones of fusion hybrid progeny. In this report, we modified the cancer-stromal coculture
Esperanza Martín-Sánchez et al.
PloS one, 9(11), e112148-e112148 (2014-11-12)
Currently, there is no efficient therapy for patients with peripheral T cell lymphoma (PTCL). The Proviral Integration site of Moloney murine leukemia virus (PIM) kinases are important mediators of cell survival. We aimed to determine the therapeutic value of PIM
Two-Photon Microscopy Analysis of Gold Nanoparticle Uptake in 3D Cell Spheroids.
Rane TD and Armani AM
PLoS ONE, 11(12) (2016)
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The androgen receptor (AR) pathway plays a central role in prostate cancer (PCa) growth and progression and is a validated therapeutic target. In response to ligand binding AR translocates to the nucleus, though the molecular mechanism is not well understood.
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文章

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

提高慢病毒轉導效率的方法

比較了慢病毒轉導 Jurkat 細胞的方法。比較了 Spinoculation 與 polybrene (hexadimethrine bromide) 及 fibronection 塗佈板的過夜孵育。

條款

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

慢病毒轉導協議

如何進行 MISSION shRNA 慢病毒顆粒的慢病毒轉導，以達到長期穩定的沉默和表型改變的詳細程序。

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