推薦產品
產品線
MISSION®
濃度
≥1x106 VP/ml (via p24 assay)
技術
capture ELISA: 106 TU/mL using p24
運輸包裝
dry ice
儲存溫度
−70°C
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一般說明
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
應用
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors. It has also been used to generate fluorescent cell lines.
MISSION®pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction. It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP.
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012).
This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.
This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.
法律資訊
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.
推薦
產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Albin Rudisch et al.
PloS one, 10(4), e0124283-e0124283 (2015-04-29)
We established co-cultures of invasive or non-invasive NSCLC cell lines and various types of fibroblasts (FBs) to more precisely characterize the molecular mechanism of tumor-stroma crosstalk in lung cancer. The HGF-MET-ERK1/2-CREB-axis was shown to contribute to the onset of the
High EMT signature score of invasive non-small cell lung cancer (NSCLC) cells correlates with NF kappa B driven colony-stimulating factor 2 (CSF2/GM-CSF) secretion by neighboring stromal fibroblasts
Rudisch A, et al.
PLoS ONE, 10(4), e0124283-e0124283 (2015)
Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake
Jeong JH, et al.
PLoS Biology, 16(4), e2004399-e2004399 (2018)
A high-content image-based method for quantitatively studying context-dependent cell population dynamics.
Garvey CM
Scientific Reports, 6, 29752-29752 (2016)
Jae Hoon Jeong et al.
PLoS biology, 16(4), e2004399-e2004399 (2018-04-25)
Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC) respond to numerous hormonal and neural signals, resulting in changes in food intake. Here, we demonstrate that ARC POMC neurons express capsaicin-sensitive transient receptor potential vanilloid 1 receptor (TRPV1)-like
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