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表单
powder
比活
≥1,500 NIH units/mg protein (E1%/280 = 19.5)
分子量
heavy chain ~31 kDa
light chain ~6 kDa
技术
cell culture | mammalian: suitable
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
cow ... F2(280685)
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一般描述
凝血酶是止血过程中的最终凝血蛋白酶，可同时促进促凝血和抗凝血作用。
应用
凝血酶用于位点融合蛋白的位点特异性裂解，重组融合蛋白含有可进入的凝血酶识别位点，用于去除亲和标签。在一项研究中，凝血酶被用于评估用于生物物理学和结构表征的腺苷受体肽生物合成的表达和纯化系统。 [1]
生化/生理作用
丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。
单位定义
活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比来测定。
重悬
当使用 1mL 水复原时，小瓶将在 0.15M 氯化钠和 0.05M 柠檬酸钠中以及 pH 6.5 下达到规定的活性。
分析说明
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。
其他说明
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
底物
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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