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Merck
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文件

B7632

Sigma-Aldrich

N-苯甲酰基-Phe-Val-Arg-对硝基苯胺 盐酸盐

chromogenic, protease substrate, ≥98% (TLC), powder

别名:

N-苯甲酰基-L-苯丙氨酰-L-缬氨酰-L-精氨酸-4-硝基苯胺 盐酸盐

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About This Item

经验公式（希尔记法）:
C33H40N8O6 · HCl
CAS号:
54799-93-8
分子量:
681.18
Beilstein:
3027332
MDL號碼:
MFCD00038397
分類程式碼代碼:
12352204
PubChem物質ID:
24892011
NACRES:
NA.32

product name

N-苯甲酰基-Phe-Val-Arg-对硝基苯胺 盐酸盐, protease substrate

化驗

≥98% (TLC)

形狀

powder

溶解度

methanol: 20 mg/mL, clear, colorless to light yellow

儲存溫度

−20°C

SMILES 字串

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI 密鑰

PYVSMZDQQUZGPF-JAQKLANPSA-N

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一般說明

N-苯甲酰基-Phe-Val-Arg-对硝基苯胺是显色蛋白酶底物。

應用

  • N

  • -苯甲酰基-Phe-Val-Arg-对硝基苯胺盐酸盐已作为底物：用于凝血酶菌丝
  • 的可溶性和颗粒部分中的胰蛋白酶样酶，来自蛇毒的重组和天然巴曲酶
  • 用于酰胺分解活性测定中的纤溶酶aprE2

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

基底

胰蛋白酶，凝血酶和蛇毒凝血酶的底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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I J Mackie et al.
Thrombosis research, 28(4), 499-507 (1982-11-15)
The Factor VIII content of Factor IX concentrates was investigated by agarose gel electrophoresis which removed the interfering effects of stabilisers and proteolytic enzyme inhibitors. Factor VIII coagulant activity (FVIII C) as measured by clotting and amidolytic methods correlated well
The hydrolysis of N-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide and its use as a substrate for the assay of cathepsin B.
J Butterworth et al.
Analytical biochemistry, 106(1), 156-162 (1980-07-15)
Maxsuell Lucas Mendes Marques et al.
Marine drugs, 17(1) (2018-12-24)
In this study, sulfated polysaccharide-rich extracts were isolated from 22 tropical seaweeds (4 red, 11 brown, and 7 green) found in northeastern Brazil, and evaluated for the role of anticoagulant agents. Fifteen of the extracts showed anticoagulant activity, including all
Heparin and protease inhibition. II. The role of heparin in the ATIII inactivation of thrombin, plasmin, and trypsin.
G F Smith et al.
Thrombosis research, 22(1-2), 115-133 (1981-04-01)
Inhibition of human coagulation factor Xa by thrombin substrates.
C L Orthner et al.
Thrombosis research, 23(6), 533-539 (1981-09-15)
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实验方案

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

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