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Merck
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文件

S2015

Sigma-Aldrich

SSPE 缓冲液 20× 浓缩液

for Northern and Southern blotting, solution

别名:

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

等級

for molecular biology

品質等級

200

無菌

0.2 μm filtered

形狀

solution

異物活動

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

儲存溫度

room temp

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相关类别

一般說明

SSPE Buffer 20x 液体是南方和北方杂交程序中的标准试剂。该液体可根据需要直接使用或稀释。

應用

适用于：
  • Northern 和 Southern 印迹杂交缓冲液
  • 微阵列的清洗缓冲液

特點和優勢

  • 方便的即用型液体
  • 杂交中的多用途

成分

SSPE Buffer 20x 在 0.2M 磷酸盐缓冲液 (pH 7.4) 中含有 0.02M EDTA 和 2.98M NaCl。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
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Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Claudia S Meier-Trummer et al.
PloS one, 4(7), e6265-e6265 (2009-07-16)
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

实验方案

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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