This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
推荐产品
产品名称
杜氏磷酸盐缓冲盐水, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
sodium pyruvate: no
glucose: no (Dextro)
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
杜氏磷酸盐缓冲盐水（D-PBS）是一种用于组织培养的平衡盐溶液。
應用
D-PBS是通用的等渗盐水溶液，用于在制备和连续转移过程中洗涤细胞。
杜氏磷酸盐缓冲盐水（D-PBS）已用于：
- 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
- 在Caco-2BBe细胞经上皮电阻（TEER）测量后清洗和平衡细胞
- 清洗细胞以进行蛋白质分离
生化／生理作用
杜氏磷酸盐缓冲盐水（D-PBS）有四种主要用途：
- 为细胞提供正常细胞代谢所必需的水分和一定体积的无机离子
- 充当灌溉、运输和稀释液体，同时维持细胞内和细胞外渗透平衡
- 与碳水化合物（如葡萄糖）结合时，为细胞代谢提供主要的能量来源
- 提供缓冲系统，以维持培养基的生理pH值范围
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Anna Eskilsson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(19), 5035-5044 (2017-04-26)
Fever occurs upon binding of prostaglandin E
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
实验方案
制备3D癌症干细胞球状体的分步肿瘤成球实验方案
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
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What is the concentration of MgCl2 and CaCl2?
1 answer-
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¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
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