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Merck

67996

Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

形狀

solution

品質

50x Concentrate

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值

8.3±0.2 (25 °C)

密度

1.09 g/mL at 20 °C

儲存溫度

room temp

一般說明

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

應用

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

成分

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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